caa a22 4500 463160495 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0138-1 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0138-1 Plantlet regeneration from fascicular buds of seedling shoot apices of Pinus roxburghii Sarg [Elektronische Daten] [R. Kalia, S. Arya, S. Kalia, I. Arya] Shoot apices of Pinus roxburghii Sarg were cultured on Murashige and Skoog's medium (MS) supplemented with cytokinins [6-benzyladenine (BA), kinetin and N-benzyl-9-(2-tetrahydropyranyl) adenine (BPA)] alone and in combination with auxin, α-napthaleneacetic acid (NAA). Of the three cytokinins tested at varying concentrations, medium supplemented with 10 µM BA was found optimal in respect of explant responsiveness (97.22 %) and average number of buds induced per explant (7.42). The concentration of cytokinins in the induction medium had a profound effect on rate of elongation of induced buds on MS basal medium containing 0.5 % activated charcoal. Further, shoots induced on lower concentrations of BA increased up to 2.4 times in length in 4 weeks. Decapitation of the explant enhanced the rate of axillary bud elongation. Proliferating shoot cultures were established by sub-culturing the axillary shoots on MS supplemented with 10 µM BA. Shoots 2-3 cm in length were suitable for culturing as more buds were induced on them compared to longer or shorter shoots. Root primordia were induced on 70.83 % shoots when transferred to 1/2 MS medium supplemented with 5.0 µM NAA. Elongation of root primordia (60 %) was achieved in liquid 1/2 MS basal medium. The plantlets were successfully transferred to soil after hardening; the time period from initiation of shoot buds to transplantation being 20-22 weeks. Institute of Experimental Botany, ASCR, 2007 apical bud nationallicence auxins nationallicence axillary bud nationallicence chir pine nationallicence cytokinins nationallicence micropropagation nationallicence seedling nationallicence BA : 6-benzyladenine nationallicence BPA : N-benzyl-9-(2-tetrahydropyranyl) adenine nationallicence IAA : indole-3-acetic acid nationallicence IBA : indole-3-butyric acid nationallicence MS : Murashige and Skoog's medium (1962) nationallicence NAA : α-napthaleneacetic acid nationallicence Kalia R. Tissue Culture Laboratory, Division of Genetics and Tree Propagation, Forest Research Institute, 248006, Dehradun, Uttaranchal, India aut Arya S. Tissue Culture Laboratory, Division of Genetics and Tree Propagation, Forest Research Institute, 248006, Dehradun, Uttaranchal, India aut Kalia S. Tissue Culture Laboratory, Division of Genetics and Tree Propagation, Forest Research Institute, 248006, Dehradun, Uttaranchal, India aut Arya I. Tissue Culture Laboratory, Division of Genetics and Tree Propagation, Forest Research Institute, 248006, Dehradun, Uttaranchal, India aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 653-659 0006-3134 51:4<653 2007 51 10535 https://doi.org/10.1007/s10535-007-0138-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0138-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kalia R. Tissue Culture Laboratory, Division of Genetics and Tree Propagation, Forest Research Institute, 248006, Dehradun, Uttaranchal, India aut NATIONALLICENCE 700 1- Arya S. Tissue Culture Laboratory, Division of Genetics and Tree Propagation, Forest Research Institute, 248006, Dehradun, Uttaranchal, India aut NATIONALLICENCE 700 1- Kalia S. Tissue Culture Laboratory, Division of Genetics and Tree Propagation, Forest Research Institute, 248006, Dehradun, Uttaranchal, India aut NATIONALLICENCE 700 1- Arya I. Tissue Culture Laboratory, Division of Genetics and Tree Propagation, Forest Research Institute, 248006, Dehradun, Uttaranchal, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 653-659 0006-3134 51:4<653 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer