caa a22 4500 463160517 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0149-y doi (NATIONALLICENCE)springer-10.1007/s10535-007-0149-y In vitro selection of salt tolerant cell lines in Solanum tuberosum L [Elektronische Daten] [F. Queirós, F. Fidalgo, I. Santos, R. Salema] Cell lines able to grow on media containing 50, 100, 150 or 200 mM NaCl were established from potato callus cultures by direct recurrent selection or gradual selection. In callus subjected to direct selection only small clusters of cells survived on medium with 150 or 200 mM NaCl, whereas on 100 mM small cell portions appear necrotic. When cell lines were obtained by successive subcultures on media with increased concentrations of NaCl, salt-tolerant calli were more compact and developed a greenish colour free from necrotic areas. The response of calli lines grown on media with NaCl was compared to control line. The NaCl-tolerant calli showed a decrease in relative growth rate and water content, with higher reductions in the 150 mM tolerant callus. Lipid peroxidation was increased in 50 mM and 100 mM NaCl-tolerant calli, while in 150 mM tolerant callus remained similar to 100 mM values. There was a significant increase in ascorbic acid content in 100 mM and 150 mM NaCl-tolerant calli as compared to the 50 mM, that was two-fold the value found in the control. Also, the contents of soluble and insoluble proteins increased in salt-tolerant lines. SDS-PAGE of soluble proteins showed the synthesis of specific polypeptides in the presence of NaCl in culture medium and the synthesis of a new polypeptide. Institute of Experimental Botany, ASCR, 2007 ascorbic acid nationallicence callus tissue nationallicence lipid peroxidation nationallicence NaCl tolerance nationallicence potato nationallicence proteins nationallicence salt stress nationallicence AsA : ascorbic acid nationallicence BA : benzylaminopurine nationallicence MDA : malondialdehyde nationallicence ROS : reactive oxygen species nationallicence SDS-PAGE : sodium dodecyl sulphate-polyacrylamide gel electrophoresis nationallicence TCA : trichloroacetic acid nationallicence Queirós F. Departamento de Botânica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal aut Fidalgo F. Departamento de Botânica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal aut Santos I. Departamento de Botânica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal aut Salema R. Departamento de Botânica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 728-734 0006-3134 51:4<728 2007 51 10535 https://doi.org/10.1007/s10535-007-0149-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0149-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Queirós F. Departamento de Botânica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal aut NATIONALLICENCE 700 1- Fidalgo F. Departamento de Botânica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal aut NATIONALLICENCE 700 1- Santos I. Departamento de Botânica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal aut NATIONALLICENCE 700 1- Salema R. Departamento de Botânica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 728-734 0006-3134 51:4<728 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer