caa a22 4500 463160533 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0137-2 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0137-2 Paclitaxel and baccatin III production induced by methyl jasmonate in free and immobilized cells of Taxus baccata [Elektronische Daten] [M. Bonfill, S. Bentebibel, E. Moyano, J. Palazón, R. Cusidó, R. Eibl, M. Piñol] The effects of 100 and 200 µM methyl jasmonate (MJA) on cell proliferation and paclitaxel and baccatin III production were investigated in free and alginate immobilized cells of Taxus baccata growing in a selected product formation culture medium. The greatest accumulation of paclitaxel (13.20 mg dm−3) and baccatin III (4.62 mg dm−3) occurred when 100 µM MJA was added to the culture medium of cells entrapped using a 1.5 and 2.5 % alginate solution. The effects of different treatments on the viability of cultured cells and their capacity to excrete both taxanes into the surrounding medium were considered. Institute of Experimental Botany, ASCR, 2007 alginate entrapped cells nationallicence cell cultures nationallicence elicitation nationallicence taxanes nationallicence yew nationallicence B5 : Gamborg's B5 medium nationallicence BAP : benzylaminopurine nationallicence MJA : methyl jasmonate nationallicence NAA : naphthaleneacetic acid nationallicence Bonfill M. Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028, Barcelona, Spain aut Bentebibel S. Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028, Barcelona, Spain aut Moyano E. Departament de Ciències Experimentals i de Salut, Universitat Pompeu Fabra, E-08003, Barcelona, Spain aut Palazón J. Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028, Barcelona, Spain aut Cusidó R. Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028, Barcelona, Spain aut Eibl R. Hochschule Wädenswill, CH-8820, Wädenswill, Switzerland aut Piñol M. Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028, Barcelona, Spain aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 647-652 0006-3134 51:4<647 2007 51 10535 https://doi.org/10.1007/s10535-007-0137-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0137-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bonfill M. Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Bentebibel S. Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Moyano E. Departament de Ciències Experimentals i de Salut, Universitat Pompeu Fabra, E-08003, Barcelona, Spain aut NATIONALLICENCE 700 1- Palazón J. Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Cusidó R. Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Eibl R. Hochschule Wädenswill, CH-8820, Wädenswill, Switzerland aut NATIONALLICENCE 700 1- Piñol M. Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028, Barcelona, Spain aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 647-652 0006-3134 51:4<647 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer