caa a22 4500 463160541 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0160-3 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0160-3 In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds [Elektronische Daten] [M. Sujatha, V. Dinesh Kumar] The organogenic competence of leaf explants of eleven Carthamus species including C. tinctorius on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) + α-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) + NAA was investigated. Highly prolific adventitious shoot regeneration was observed in C. tinctorius and C. arborescens on both growth regulator combinations and the shoot regeneration frequency was higher on medium supplemented with TDZ + NAA. Nodal culture of nine Carthamus species on media supplemented with BA and kinetin (KIN) individually revealed the superiority of media supplemented with BA over that of KIN in facilitating a higher shoot proliferation index. Proliferating shoots from axillary buds and leaf explants were transferred to medium supplemented with 1.0 mg dm−3 KIN or 0.5 mg dm−3 BA for shoot elongation. Elongated shoots were rooted on half-strength MS medium supplemented with 1.0 mg dm−3 each of indole-butyric acid (IBA) and phloroglucinol. The plantlets thus obtained were hardened and transferred to soil. Institute of Experimental Botany, ASCR, 2007 direct organogenesis nationallicence growth regulators nationallicence in vitro culture nationallicence tissue culture nationallicence wild safflowers nationallicence BA : 6-benzyladenine nationallicence IBA : indole-butyric acid nationallicence KIN : kinetin nationallicence NAA : α-naphthaleneacetic acid nationallicence TDZ : thidiazuron nationallicence Sujatha M. Crop Improvement Section, Directorate of Oilseeds Research, Rajendranagar, 500030, Hyderabad, India aut Dinesh Kumar V. Crop Improvement Section, Directorate of Oilseeds Research, Rajendranagar, 500030, Hyderabad, India aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 782-786 0006-3134 51:4<782 2007 51 10535 https://doi.org/10.1007/s10535-007-0160-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0160-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sujatha M. Crop Improvement Section, Directorate of Oilseeds Research, Rajendranagar, 500030, Hyderabad, India aut NATIONALLICENCE 700 1- Dinesh Kumar V. Crop Improvement Section, Directorate of Oilseeds Research, Rajendranagar, 500030, Hyderabad, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 782-786 0006-3134 51:4<782 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer