caa a22 4500 463160649 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0048-2 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0048-2 Agrobacterium -mediated transformation of Cry1C, Cry2A and Cry9C genes into Gossypium hirsutum and plant regeneration [Elektronische Daten] [X. Guo, C. Huang, S. Jin, S. Liang, Y. Nie, X. Zhang] Three constructs harbouring novel Bacillus thuringiensis genes (Cry1C, Cry2A, Cry9C) and bar gene were transformed into four upland cotton cultivars, Ekangmian10, Emian22, Coker201 and YZ1 via Agrobacterium-mediated transformation. With the bar gene as a selectable marker, about 84.8 % of resistant calli have been confirmed positive by polymerase chain reaction (PCR) tests, and totally 50 transgenic plants were regenerated. The insertions were verified by means of Southern blotting. Bioassay showed 80 % of the transgenic plantlets generated resistance to both herbicide and insect. We optimized conditions for improving the transformation efficiency. A modified in vitro shoot-tip grafting technique was introduced to help entire transplantation. This result showed that bar gene can replace antibiotic marker genes (ex. npt II gene) used in cotton transformation. Institute of Experimental Botany, ASCR, 2007 Agrobacterium tumefaciens nationallicence bar gene nationallicence cotton nationallicence novel Bt genes nationallicence AS : acetosyringone nationallicence EC : embryogenic callus nationallicence IBA : indole-3-butyric acid nationallicence npt II : neomycin phosphotransferase nationallicence L-L-PPT : L-phosphinothricin nationallicence AS : acetosyringone nationallicence PCR : polymerase chain reaction nationallicence MSB : Murashige and Skoog (1962) medium with B5 (Gamborg et al. 1968) vitamins nationallicence PAT : phosphinothricin acetyltransferase nationallicence Guo X. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Huang C. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Jin S. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Liang S. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Nie Y. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Zhang X. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 242-248 0006-3134 51:2<242 2007 51 10535 https://doi.org/10.1007/s10535-007-0048-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0048-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guo X. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Huang C. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Jin S. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Liang S. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Nie Y. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Zhang X. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 242-248 0006-3134 51:2<242 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer