caa a22 4500 463160665 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0050-8 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0050-8 Identification and sequence analysis of cDNA fragments relative to ovary development of Cymbidium hybridium after pollination [Elektronische Daten] [X. Chen, C. Wang, Y. Zhang, W. Song, R. Chen] In this study, seven cDNA fragments of genes, differentially expressed in ovaries after pollination in Cymbidium hybridium, were identified and characterized by mRNA differential display reverse transcription polymerase chain reaction (DDRT-PCR). Four (CDD-313, CDD-272, CDD-265, CDD-243) among these seven cDNA fragments showed no significant homology with ESTs or genes in the databases of NCBI; another three (CDD-193, CDD-218, CDD-470) showed significant homologies with sequences encoding components of an ABC-type transporter, a GTPase and 40S ribosomal S3 proteins (RPS3), respectively. The differential expression patterns of them were confirmed by reverse Northern dot blot analysis. More interestingly, CDD-470 appeared to be present and highly expressed in the pollinated ovaries and encoded a new factor of RPS3 participating in cell growth and proliferation. We deduced that this 40s ribosomal S3 like protein was involved in ovary development of orchids. Institute of Experimental Botany, ASCR, 2007 differential display RT-PCR nationallicence floral development nationallicence 40S ribosomal S3 protein (RPS3) nationallicence BLAST : basic local alignment search tool nationallicence DDRT-PCR : differential display RT-PCR nationallicence PAGE : polyacrylamide gel electrophoresis nationallicence PCR : polymerase chain reaction nationallicence RPS3 : 40s ribosomal S3 proteins nationallicence RT-PCR : reverse transcription-PCR nationallicence Chen X. College of Life Sciences, Nankai University, 300071, Tianjin, China aut Wang C. College of Life Sciences, Nankai University, 300071, Tianjin, China aut Zhang Y. College of Life Sciences, Nankai University, 300071, Tianjin, China aut Song W. College of Life Sciences, Nankai University, 300071, Tianjin, China aut Chen R. College of Life Sciences, Nankai University, 300071, Tianjin, China aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 249-256 0006-3134 51:2<249 2007 51 10535 https://doi.org/10.1007/s10535-007-0050-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0050-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen X. College of Life Sciences, Nankai University, 300071, Tianjin, China aut NATIONALLICENCE 700 1- Wang C. College of Life Sciences, Nankai University, 300071, Tianjin, China aut NATIONALLICENCE 700 1- Zhang Y. College of Life Sciences, Nankai University, 300071, Tianjin, China aut NATIONALLICENCE 700 1- Song W. College of Life Sciences, Nankai University, 300071, Tianjin, China aut NATIONALLICENCE 700 1- Chen R. College of Life Sciences, Nankai University, 300071, Tianjin, China aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 249-256 0006-3134 51:2<249 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer