caa a22 4500 463160681 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0047-3 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0047-3 Ginsenoside production, growth and cytogenetic characteristics of sustained Panax japonicus var. Repens cell suspension culture [Elektronische Daten] [I. Smolenskaya, O. Reshetnyak, A. Nosov, S. Zoriniants, A. Chaiko, Y. Smirnova, A. Nosov] Cytophysiological and cytogenetic characteristics of cell suspension culture of Panax japonicus var. repens were studied in relation to the accumulation of ginsenosides (GSs). The minimal time of cell number doubling was 1.3 ± 0.1 d and cell number increased 7 to 8-fold during growth cycle. The cell culture can be considered as aneuploid with about tetraploid (46-60 chromosomes) modal class. Upon long-term cultivation, the total content of GSs considerably increased and maximal concentration of GSs was 2.2 %(d.m.). The ratio of seven major GSs only slightly altered both over each and different subcultures. The overall amount of GSs of Rg-group significantly exceeded that of Rb-group. Cell volume and the number of large cellular aggregates with the higher proportion (by 20 %) of parenchymal cells increased late in the subculture. In this time the population contained about 20 % of the cells with doubled amount of nuclear DNA and accompanied with elevation in the GS content. These data prompted us to suggest that biosynthesis of GSs has a link with cell differentiation. Institute of Experimental Botany, ASCR, 2007 chromosome number nationallicence cytodifferentiation nationallicence ginseng nationallicence in vitro culture nationallicence nuclear DNA content nationallicence triterpenes nationallicence HPLC : high-performance liquid chromatography nationallicence GS : ginsenoside nationallicence PJ : Panax japonicus var. repens nationallicence SM : secondary metabolite nationallicence Smolenskaya I. Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut Reshetnyak O. Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut Nosov A. Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut Zoriniants S. Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut Chaiko A. Institute of Food Chemistry and Technology, Ukrainian National Academy of Sciences and Ministry of Agricultural Politics, Osypovskogo 2a, 04123, Kyiv, Ukraine aut Smirnova Y. Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 235-241 0006-3134 51:2<235 2007 51 10535 https://doi.org/10.1007/s10535-007-0047-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0047-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smolenskaya I. Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut NATIONALLICENCE 700 1- Reshetnyak O. Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut NATIONALLICENCE 700 1- Nosov A. Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut NATIONALLICENCE 700 1- Zoriniants S. Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut NATIONALLICENCE 700 1- Chaiko A. Institute of Food Chemistry and Technology, Ukrainian National Academy of Sciences and Ministry of Agricultural Politics, Osypovskogo 2a, 04123, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Smirnova Y. Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 235-241 0006-3134 51:2<235 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer