caa a22 4500 463160703 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0072-2 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0072-2 Effects of brassinosteroid infiltration prior to cold treatment on ion leakage and pigment contents in rape leaves [Elektronische Daten] [A. Janeczko, G. Gullner, A. Skoczowski, F. Dubert, B. Barna] The effect of 24-epibrassinolide (BR27) on cold resistance of rape seedlings was studied by ion leakage and photosynthetic pigment degradation measurements. Aqueous solutions of BR27 were injected into cotyledons or primary leaves of rape plants and these plants were incubated at 2 °C or 20 °C. Cold treatment (2 °C) without BR27 injection elevated the membrane permeability in both primary leaves and cotyledons significantly. Surprisingly, injection of leaves with water or 0.467 % aqueous ethanol solution led to a massive increase in membrane permeability after cold stress at 2 °C. The synergistic effect of leaf infiltration and cold on permeability was abolished by 0.05 and 1.00 µM of BR27 in primary leaves and by 1.00 µM of BR27 in cotyledons. On the other hand, BR27 solutions strongly elevated the membrane permeability at 20 °C, while water and ethanol solutions brought about only negligible increases. Water or ethanol infiltrations strongly reduced the leaf contents of chlorophyll (Chl) a, Chl b and carotenoids at 2 °C but less markedly at 20 °C. However, in seedlings exposed to 2 °C pigments content was significantly higher in BR27-treated leaves as compared to water/ethanol control. There were no differences between pigment contents of leaves injected with BR27 solutions or only water/ethanol at 20 °C. The above data strongly support the stress protecting effect of BR27. Institute of Experimental Botany, ASCR, 2007 carotenoids nationallicence chlorophyll nationallicence cold stress nationallicence 24-epibrassinolide nationallicence ion leakage nationallicence BRs : brassinosteroids nationallicence BR27 : 24-epibrassinolide nationallicence PAR : photosynthetically active radiation nationallicence Janeczko A. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 21 Niezapominajek St., PL-30239, Krakow, Poland aut Gullner G. Plant Protection Institute, Hungarian Academy of Sciences, 15 Herman Otto St., H-1022, Budapest, Hungary aut Skoczowski A. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 21 Niezapominajek St., PL-30239, Krakow, Poland aut Dubert F. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 21 Niezapominajek St., PL-30239, Krakow, Poland aut Barna B. Plant Protection Institute, Hungarian Academy of Sciences, 15 Herman Otto St., H-1022, Budapest, Hungary aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 355-358 0006-3134 51:2<355 2007 51 10535 https://doi.org/10.1007/s10535-007-0072-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0072-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Janeczko A. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 21 Niezapominajek St., PL-30239, Krakow, Poland aut NATIONALLICENCE 700 1- Gullner G. Plant Protection Institute, Hungarian Academy of Sciences, 15 Herman Otto St., H-1022, Budapest, Hungary aut NATIONALLICENCE 700 1- Skoczowski A. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 21 Niezapominajek St., PL-30239, Krakow, Poland aut NATIONALLICENCE 700 1- Dubert F. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 21 Niezapominajek St., PL-30239, Krakow, Poland aut NATIONALLICENCE 700 1- Barna B. Plant Protection Institute, Hungarian Academy of Sciences, 15 Herman Otto St., H-1022, Budapest, Hungary aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 355-358 0006-3134 51:2<355 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer