caa a22 4500 463160738 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0041-9 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0041-9 Protein patterns associated with Pisum sativum somatic embryogenesis [Elektronische Daten] [M. Griga, J. Horáček, H. Klenotičová] Total protein patterns were studied in the course of development of pea somatic embryos using simple protocol of direct regeneration from shoot apical meristems on auxin supplemented medium. Protein content and total protein spectra (SDS-PAGE) of somatic embryos in particular developmental stages were analysed in Pisum sativum, P. arvense, P. elatius and P. jomardi. Expression of seed storage proteins in somatic embryos was compared with their accumulation in zygotic embryos of selected developmental stages. Pea vegetative tissues, namely leaf and root, were used as a negative control not expressing typical seed storage proteins. The biosynthesis and accumulation of seed storage proteins was observed during somatic embryo development (since globular stage), despite of the fact that no special maturation treatment was applied. Major storage proteins typical for pea seed (globulins legumin, vicilin, convicilin and their subunits) were detected in somatic embryos. In general, the biosynthesis of storage proteins in somatic embryos was lower as compared to mature dry seed. However, in some cases the cotyledonary somatic embryos exhibited comparatively high expression of vicilin, convicilin and pea seed lectin, which was even higher than those in immature but morphologically fully developed zygotic embryos. Desiccation treatments did not affect the protein content of somatic embryos. The transfer of desiccated somatic embryos on hormone-free germination medium led to progressive storage protein degradation. The expression of true seed storage proteins may serve as an explicit marker of somatic embryogenesis pathway of regeneration as well as a measure of maturation degree of somatic embryos in pea. Institute of Experimental Botany, ASCR, 2007 embryo development nationallicence pea nationallicence seed proteins nationallicence storage substances nationallicence zygotic embryogenesis nationallicence MSB-medium : medium with mineral salts after Murashige and Skoog (1962) and vitamins after Gamborg et al. (1968) nationallicence SDS-PAGE : sodium dodecylsulphate polyacrylamide gel electrophoresis nationallicence SE : somatic embryo nationallicence ZE : zygotic embryo nationallicence Griga M. Plant Biotechnology Department, AGRITEC Ltd., Zemědělská 16, CZ-78701, Šumperk, Czech Republic aut Horáček J. Plant Biotechnology Department, AGRITEC Ltd., Zemědělská 16, CZ-78701, Šumperk, Czech Republic aut Klenotičová H. Plant Biotechnology Department, AGRITEC Ltd., Zemědělská 16, CZ-78701, Šumperk, Czech Republic aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 201-211 0006-3134 51:2<201 2007 51 10535 https://doi.org/10.1007/s10535-007-0041-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0041-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Griga M. Plant Biotechnology Department, AGRITEC Ltd., Zemědělská 16, CZ-78701, Šumperk, Czech Republic aut NATIONALLICENCE 700 1- Horáček J. Plant Biotechnology Department, AGRITEC Ltd., Zemědělská 16, CZ-78701, Šumperk, Czech Republic aut NATIONALLICENCE 700 1- Klenotičová H. Plant Biotechnology Department, AGRITEC Ltd., Zemědělská 16, CZ-78701, Šumperk, Czech Republic aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 201-211 0006-3134 51:2<201 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer