caa a22 4500 463160797 CHVBK 20180406164747.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0055-3 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0055-3 Improvement of protein quality in transgenic soybean plants [Elektronische Daten] [H. El-Shemy, M. Khalafalla, K. Fujita, M. Ishimoto] Glycinin is one of the abundant storage proteins in soybean seeds. A modified Gy1 (A1aB1b) proglycinin gene with a synthetic DNA encoding four continuous methionines (V3-1) was connected between the hpt gene and the modified green fluorescent protein sGFP(S65T) gene, and a resultant plasmid was introduced into soybean by particle bombardment in order to improve nutritional value of its seeds. After the selection with hygromycin, the efficiency of gene introduction was evaluated. More than 60 % of the regenerated plants tolerant to hygromycin yielded the hpt and V3-1 fragment by polymerase chain reaction (PCR) analysis, and the expression of sGFP was detected in about 50 % of putative transgenic soybeans. Southern hybridization confirmed the presence of transgenes in T0 plants and the transgenic soybeans hybridized with the hpt and V3-1 genes were analyzed showed different banding patterns. Most of the transgenic plants were growing, flowering normally and produced seeds. Analysis of seed obtained from transgenic soybean plants expressing hpt and V3-1 genes showed higher accumulation of glycinin compared with non-transgenic plants. In addition, protein expression in transgenic soybean plants was observed by using 2D-electrophoresis. Institute of Experimental Botany, ASCR, 2007 genetic improvement nationallicence Glycine max nationallicence glycinin nationallicence particle bombardment nationallicence PCR nationallicence seed storage proteins nationallicence GFP : green fluorescent protein nationallicence PCR : polymerase chain reaction nationallicence El-Shemy H. Biochemistry Department, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt aut Khalafalla M. National Agricultural Research Center for Western Region, 721-8514, Fukuyama, Hiroshima, Japan aut Fujita K. Graduate School of Biosphere Sciences, Hiroshima University, 739-8528, Higashi-Hiroshima, Japan aut Ishimoto M. National Agricultural Research Center for Western Region, 721-8514, Fukuyama, Hiroshima, Japan aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 277-284 0006-3134 51:2<277 2007 51 10535 https://doi.org/10.1007/s10535-007-0055-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0055-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- El-Shemy H. Biochemistry Department, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt aut NATIONALLICENCE 700 1- Khalafalla M. National Agricultural Research Center for Western Region, 721-8514, Fukuyama, Hiroshima, Japan aut NATIONALLICENCE 700 1- Fujita K. Graduate School of Biosphere Sciences, Hiroshima University, 739-8528, Higashi-Hiroshima, Japan aut NATIONALLICENCE 700 1- Ishimoto M. National Agricultural Research Center for Western Region, 721-8514, Fukuyama, Hiroshima, Japan aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 277-284 0006-3134 51:2<277 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer