caa a22 4500 463160819 CHVBK 20180406164747.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0046-4 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0046-4 Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana [Elektronische Daten] [J. Wu, Z. Zhao, H. Xing, H. Guo, W. Li, L. An, S. Xu, T. Chen] The influence of freezing treatment on plasma membrane (PM) H+-ATPase was investigated using plasma membrane vesicles isolated from calluses from Chorispora bungeana Fisch. & C.A. Mey. by the discontinuous sucrose gradient centrifugation. Freezing treatment (−4 °C) for 5 d resulted in significant increases in the ATPase activity and the activity of p-nitrophenyl phosphate (PNPP) hydrolysis, decreases in the Km for ATP hydrolysis and PNPP hydrolysis, and the shift of optimal pH from 6.5 to 7.0. Also, the activity PNPP hydrolysis was less sensitive to vanadate after freezing treatment compared to control, while the inhibition of ATP hydrolysis by hydroxylamine was more sensitive. In addition, freezing treatment also decreased the activation effects of trypsin on PNPP hydrolysis, but increased the activation effects of lysophosphatidylcholine on ATP hydrolysis. Taken together, these results suggested that PM H+-ATPase might play an important role during adaptation to freezing and enhancing the frost hardness in C. bungeana. Institute of Experimental Botany, ASCR, 2007 alpine plant nationallicence kinase domain nationallicence p -nitrophenyl phosphate nationallicence phosphatase domain nationallicence ATP : adenosine triphosphate nationallicence BSA : bovine serum albumin nationallicence DTT : dithiothreitol nationallicence EDTA : ethylenediaminetetraacetic acid nationallicence lyso-PC : lysophosphatidylcholine nationallicence Mes : 2-(N-morpholino)-ethanesulfonic acid nationallicence PM : plasma membrane nationallicence PMSF : phenylmethylsulfonyl fluoride nationallicence PNPP : p-nitrophenyl phosphate nationallicence Tris : N-tris(hydroxymethyl)-amino methane nationallicence Wu J. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut Zhao Z. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut Xing H. College of Agronomy, Gansu Agriculture University, 730070, Gansu, Lanzhou, P.R. China aut Guo H. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut Li W. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut An L. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut Xu S. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut Chen T. Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Science, 730000, Gansu, Lanzhou, P.R. China aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 229-234 0006-3134 51:2<229 2007 51 10535 https://doi.org/10.1007/s10535-007-0046-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0046-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu J. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut NATIONALLICENCE 700 1- Zhao Z. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut NATIONALLICENCE 700 1- Xing H. College of Agronomy, Gansu Agriculture University, 730070, Gansu, Lanzhou, P.R. China aut NATIONALLICENCE 700 1- Guo H. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut NATIONALLICENCE 700 1- Li W. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut NATIONALLICENCE 700 1- An L. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut NATIONALLICENCE 700 1- Xu S. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, 730000, Gansu, Lanzhou, P.R. China aut NATIONALLICENCE 700 1- Chen T. Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Science, 730000, Gansu, Lanzhou, P.R. China aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 229-234 0006-3134 51:2<229 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer