caa a22 4500 463160878 CHVBK 20180406164747.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0077-x doi (NATIONALLICENCE)springer-10.1007/s10535-007-0077-x Soapwort oxidoreductase is involved in trinitrotoluene detoxification [Elektronische Daten] [R. Podlipná, A. Nepovím, P. Soudek, M. Vágner, T. Vaněk] Plant enzymes participating in degradation of nitroaromatic compounds have not been biochemically characterized in details so far. From suspension culture of soapwort (Saponaria officinalis L.) we isolated a novel plant oxidoreductase involved in degradation of trinitrotoluene (TNT). The enzyme catalyses first steps of reduction of TNT nitro groups in the presence of NAD(P)H under anaerobic conditions. The enzyme is monomeric with molecular mass 29 kDa, its two isoforms have pI 4.8 and 5.1. According to the spectral and activation analysis the enzyme contains flavinmono-nucleotide as a prosthetic group. The structure properties suggest an evolutional relationship to oxophytodienoate reductase. The N-terminal amino acid sequence shows homology to family of Old Yellow Enzyme (E.C. 1.6.99.1). Institute of Experimental Botany, ASCR, 2007 flavoprotein nationallicence Old Yellow Enzyme nationallicence oxidoreductase nationallicence phytoremediation nationallicence Saponaria officinalis nationallicence BAP : N 6-benzylaminopurine nationallicence 2,4-D : 2,4-dichlorphenoxyacetic acid nationallicence MS : Murashige and Skoog nationallicence TNT : trinitrotoluene nationallicence Podlipná R. Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany, Academy of Sciences of the Czech Republic and Research Institute of Crop Production, Rozvojová 263, CZ-16502, Praha 6, Czech Republic aut Nepovím A. Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany, Academy of Sciences of the Czech Republic and Research Institute of Crop Production, Rozvojová 263, CZ-16502, Praha 6, Czech Republic aut Soudek P. Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany, Academy of Sciences of the Czech Republic and Research Institute of Crop Production, Rozvojová 263, CZ-16502, Praha 6, Czech Republic aut Vágner M. Laboratory of Biologically Active Compounds, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, CZ-16502, Praha 6, Czech Republic aut Vaněk T. Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany, Academy of Sciences of the Czech Republic and Research Institute of Crop Production, Rozvojová 263, CZ-16502, Praha 6, Czech Republic aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 367-371 0006-3134 51:2<367 2007 51 10535 https://doi.org/10.1007/s10535-007-0077-x text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0077-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Podlipná R. Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany, Academy of Sciences of the Czech Republic and Research Institute of Crop Production, Rozvojová 263, CZ-16502, Praha 6, Czech Republic aut NATIONALLICENCE 700 1- Nepovím A. Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany, Academy of Sciences of the Czech Republic and Research Institute of Crop Production, Rozvojová 263, CZ-16502, Praha 6, Czech Republic aut NATIONALLICENCE 700 1- Soudek P. Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany, Academy of Sciences of the Czech Republic and Research Institute of Crop Production, Rozvojová 263, CZ-16502, Praha 6, Czech Republic aut NATIONALLICENCE 700 1- Vágner M. Laboratory of Biologically Active Compounds, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, CZ-16502, Praha 6, Czech Republic aut NATIONALLICENCE 700 1- Vaněk T. Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany, Academy of Sciences of the Czech Republic and Research Institute of Crop Production, Rozvojová 263, CZ-16502, Praha 6, Czech Republic aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 367-371 0006-3134 51:2<367 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer