caa a22 4500 463160886 CHVBK 20180406164747.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0044-6 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0044-6 Effects of benzylaminopurine and irradiance on cytokinin contents, α-tubulin gene expression and cucumber cotyledon expansion [Elektronische Daten] [Y. Li, Q. Ma] The fluctuation of endogenous cytokinins was determined in the excised cucumber (Cucumis sativa L.) cotyledons incubated with benzylaminopurine (BA) under irradiance or in darkness. The data indicated that light stimulated the cotyledon expansion compared with dark and BA further enhanced the expansion of cotyledons. However, only BA treatment markedly increased the contents of endogenous cytokinins and induced α-tubulin gene expression. Actinomycin D, a well-known inhibitor of gene transcription, strongly inhibited both light-and BA-induced cotyledon expansions and increase of endogenous cytokinin contents and α-tubulin gene expression. Colchicine, an antimicrotubular reagent, partially inhibited the cotyledon expansion without affecting the cytokinin contents. Institute of Experimental Botany, ASCR, 2007 actinomycin D nationallicence colchicine nationallicence Cucumis sativa nationallicence Act D : actinomycin D nationallicence BA : benzylaminopurine nationallicence Col : colchicine nationallicence PCR : polymerase chain reaction nationallicence Li Y. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxin Cun, Xiangshan, 100093, Beijing, China aut Ma Q. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxin Cun, Xiangshan, 100093, Beijing, China aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 217-222 0006-3134 51:2<217 2007 51 10535 https://doi.org/10.1007/s10535-007-0044-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0044-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Y. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxin Cun, Xiangshan, 100093, Beijing, China aut NATIONALLICENCE 700 1- Ma Q. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxin Cun, Xiangshan, 100093, Beijing, China aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 217-222 0006-3134 51:2<217 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer