caa a22 4500 463160932 CHVBK 20180406164747.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0061-5 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0061-5 Effects of elevated ozone on chlorophyll a fluorescence in symptomatic and asymptomatic leaves of two tomato genotypes [Elektronische Daten] [E. Degl'Innocenti, L. Guidi, G. Soldatini] Two different genotypes of Lycopersicon esculentum Mill. (cv. Cuor di Bue, O3-sensitive and line 93.1033/1, O3-resistant) were treated with a single dose of ozone (150 mm3 m−3 for 3 h). The PS 2 activity was examined by measurements of chlorophyll a fluorescence on symptomatic and asymptomatic leaves. Symptoms were evident on the 4th leaves from the bottom, in both genotypes, while the 2nd leaves of the line 93.1033/1 were asymptomatic. In these leaves, the net photosynthetic rate (PN) did not change even if the Fv/Fm ratio significantly decreased. A strong reduction in PN, mostly due to the stomatal closure, was observed in Cuor di Bue. The non photochemical quenching coefficient (qNP) and the degree of PS 2 reaction centres closure (1-qP) were higher, while the quantum efficiency of PS 2 photochemistry (ΦPS2) and quantum efficiency of excitation energy capture (Φexc.) were lower in O3 treated leaves of both genotypes. The limitation of photosynthesis was shown also by a decrease in the parameter %P, which diminished compared to controls in both genotypes. The response of the two genotypes for the energy fraction dissipated as thermal energy in the PS 2 antennae (%D) was similar. The fraction of %P remained lower during the recovery in symptomatic leaves of the resistant line as compared to the controls, whereas %X, which represents the amount of light energy that is not utilized in photochemistry or dissipated in the PS 2 antennae, significantly rose in the asymptomatic leaves of this line and in both the leaves of Cuor di Bue. From data obtained we concluded that ozone affected the plants independently on the appearance of visible symptoms of injury because the leaves without visible symptoms of both the genotypes were negatively influenced. Institute of Experimental Botany, ASCR, 2007 Lycopersicon esculentum nationallicence net photosynthetic rate nationallicence quenching analysis nationallicence ci : intercellular CO2 concentration nationallicence E : transpiration rate nationallicence ETR : electron transport rate nationallicence F0 : minimum Chl fluorescence in dark adapted state nationallicence Fm : maximum Chl fluorescence in dark adapted state nationallicence Fm′ : maximum Chl fluorescence with all PS 2 reaction centres closed in light adapted state nationallicence Fs : Chl fluorescence in steady state conditions nationallicence Fv : variable Chl fluorescence in dark adapted state nationallicence Fv′ : variable Chl fluorescence in light adapted state nationallicence Fv/Fm : photochemical PS 2 photochemistry in dark adapted state nationallicence gs : stomatal conductance to water vapour nationallicence PFD : photon flux density nationallicence PN : net photosynthetic rate nationallicence PS 2 : photosystem 2 nationallicence qNP : non-photochemical quenching nationallicence qP : photochemical quenching nationallicence Φexc : excitation capture efficiency of PS 2 nationallicence ΦPS2 : actual PS 2 efficiency nationallicence %D : fraction of light absorbed in PS 2 that is dissipated in the PS 2 antenna nationallicence %P : fraction of light absorbed that is used in photochemistry nationallicence %X : fraction of light absorbed that is not used or dissipated in the PS 2 antenna nationallicence Degl'Innocenti E. Dipartimento di Chimica e Biotecnologie Agrarie, University of Pisa, Via del Borghetto 80, I-56124, Pisa, Italy aut Guidi L. Dipartimento di Chimica e Biotecnologie Agrarie, University of Pisa, Via del Borghetto 80, I-56124, Pisa, Italy aut Soldatini G. Dipartimento di Chimica e Biotecnologie Agrarie, University of Pisa, Via del Borghetto 80, I-56124, Pisa, Italy aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 313-321 0006-3134 51:2<313 2007 51 10535 https://doi.org/10.1007/s10535-007-0061-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0061-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Degl'Innocenti E. Dipartimento di Chimica e Biotecnologie Agrarie, University of Pisa, Via del Borghetto 80, I-56124, Pisa, Italy aut NATIONALLICENCE 700 1- Guidi L. Dipartimento di Chimica e Biotecnologie Agrarie, University of Pisa, Via del Borghetto 80, I-56124, Pisa, Italy aut NATIONALLICENCE 700 1- Soldatini G. Dipartimento di Chimica e Biotecnologie Agrarie, University of Pisa, Via del Borghetto 80, I-56124, Pisa, Italy aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 313-321 0006-3134 51:2<313 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer