caa a22 4500 463160967 CHVBK 20180406164747.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0045-5 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0045-5 Embryogenesis and plant regeneration from unpollinated ovary culture of Psoralea corylifolia [Elektronische Daten] [S. Chand, A. Sahrawat] Embryogenesis and plant regeneration was achieved from callus cultures derived from unpollinated ovaries of Psoralea corylifolia L. Callus was initiated from unpollinated ovaries on Murashige and Skoog (MS) medium supplemented with 2.2 µM N 6-benzyladenine (BA) and various concentrations of α-naphthaleneacetic acid (NAA (2.7 to 10.7 µM) or 2,4-dichlorophenoxyacetic acid (2,4-D (2.3 to 9 µM) alone or in combination. Highly organized embryogenic callus induction, embryo development, proliferation and maturation were achieved on transfer of callus clumps to MS medium supplemented with NAA (0.27 µM) or 2,4-D (0.23 µM) alone or in combination with BA (2.2 to 8.8 µM). Addition of abscisic acid (ABA) (0.95 to 5.8 µM) to the medium enhanced average numbers of cotyledonary stage embryos, the maximum number (34.6 ± 0.7) being obtained on MS medium containing 0.27 µM NAA, 2.2 µM BA and 3.8 µM ABA. Embryos germinated on MS medium supplemented with BA (0 to 8.8 µM). MS medium containing gibberellic acid (GA3 (0.29 to 5.8 µM) enhanced embryo germination frequency, the highest frequency (66.7 %) occurring on MS medium containing 2.2 µM BA and 4.3 µM GA3. Effect of several concentrations (3.0 to 6.0 %) of sucrose or maltose was also observed on germination of embryos. MS medium enriched with maltose supported high frequency of embryo germination. Institute of Experimental Botany, ASCR, 2007 abscisic acid nationallicence auxins nationallicence cytokinins nationallicence gibberellic acid nationallicence gynogenic haploid nationallicence maltose nationallicence sucrose nationallicence ABA : abscisic acid nationallicence BA : N 6-benzyladenine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence GA3 : gibberellic acid nationallicence NAA : α-naphthaleneacetic acid nationallicence MS : Murashige and Skoog (1962) nationallicence MSO : MS medium without any growth regulator nationallicence Chand S. Plant Tissue Culture and Genetics Research Group, School of Life Sciences, Devi Ahilya University, Khandwa Road Campus, 452017, M.P., Indore, India aut Sahrawat A. Plant Tissue Culture and Genetics Research Group, School of Life Sciences, Devi Ahilya University, Khandwa Road Campus, 452017, M.P., Indore, India aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 223-228 0006-3134 51:2<223 2007 51 10535 https://doi.org/10.1007/s10535-007-0045-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0045-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chand S. Plant Tissue Culture and Genetics Research Group, School of Life Sciences, Devi Ahilya University, Khandwa Road Campus, 452017, M.P., Indore, India aut NATIONALLICENCE 700 1- Sahrawat A. Plant Tissue Culture and Genetics Research Group, School of Life Sciences, Devi Ahilya University, Khandwa Road Campus, 452017, M.P., Indore, India aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 223-228 0006-3134 51:2<223 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer