caa a22 4500 463160975 CHVBK 20180406164747.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0063-3 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0063-3 Dubery I. Department of Biochemistry, University of Johannesburg, Kingsway Campus, P.O. Box 524, Auckland Park 2006, South Africa aut An elicitor-and pathogen-induced cdna from potato encodes a stress-responsive cyclophilin [Elektronische Daten] [I. Dubery] Differential mRNA display was used to identify pathogen-responsive, stress-related genes in potato cell suspensions treated with salicylic acid and a cell wall-derived elicitor from Phytophthora infestans. Among the positive clones identified, one was found to be expressed at a significantly higher level in elicited cells than in control cells. DNA sequencing of this amplicon revealed high homology and identified it as a potato cyclophilin cDNA. The maximum amount of the cyclophilin mRNA was found 9 to 12 h after elicitation. Cyclophilin (CyP) mRNA synthesis was also up-regulated from 12 to 24 h in potato leaves locally infected with zoospores from Phytophthora infestans. However, untreated leaves responding systemically to the pathogen showed only a weak, delayed response at 24 h post infection. The observed accumulation of potato CyP mRNA in response to salicylic acid, P. infestans elicitor and P. infestans infection, suggest that CyPs play an important role in plant stress responses. Institute of Experimental Botany, ASCR, 2007 chaperone nationallicence Phytophthora infestans nationallicence Solanum tuberosum nationallicence stress response nationallicence CyP : cyclophilin nationallicence DDRT-PCR : differential display reverse transcription polymerase chain reaction nationallicence PPI : peptidylprolyl cis-trans isomerase nationallicence Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 327-332 0006-3134 51:2<327 2007 51 10535 https://doi.org/10.1007/s10535-007-0063-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0063-3 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Dubery I. Department of Biochemistry, University of Johannesburg, Kingsway Campus, P.O. Box 524, Auckland Park 2006, South Africa aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 327-332 0006-3134 51:2<327 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer