caa a22 4500 463161009 CHVBK 20180406164747.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10535-007-0069-x doi (NATIONALLICENCE)springer-10.1007/s10535-007-0069-x Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium [Elektronische Daten] [H. Chung, J. Chen, W. Chang] A protocol for induction of direct somatic embryogenesis, secondary embryogenesis and plant regeneration of Dendrobium cv. Chiengmai Pink was developed. Thidiazuron (TDZ) at 0.3, 1 and 3 mg dm−3 induced 5-25 % of leaf tip segments of in vitro grown plants to directly form embryos after 60 d of culture, and 1 mg dm−3 TDZ was the best treatment. Somatic embryos mostly formed from leaf surfaces near cut ends, and occasionally found on leaf tips. Higher frequency of embryogenesis was obtained in light than in darkness. During subculture, secondary embryos developed from outer cell layers of primary embryos. All combinations of NAA (0, 0.1, 1 mg dm−3) and TDZ (0, 0.3, 1, 3 mg dm−3) increased the multiplication rate of embryos. It takes about 8 months from embryo induction, plantlet formation to eventually acclimatization in greenhouse. Institute of Experimental Botany, ASCR, 2007 naphthaleneacetic acid nationallicence secondary somatic embryogenesis nationallicence thidiazuron nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence MS : Murashige and Skoog (1962) medium nationallicence NAA : naphthaleneacetic acid nationallicence TDZ : thidiazuron, 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea nationallicence Chung H. Institute of Plant and Microbial Biology, Academia Sinica, 115, Taipei, Taiwan Republic of China aut Chen J. Institute of Biotechnology, National University of Kaohsiung, 811, Kaohsiung, Taiwan Republic of China aut Chang W. Institute of Plant and Microbial Biology, Academia Sinica, 115, Taipei, Taiwan Republic of China aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 346-350 0006-3134 51:2<346 2007 51 10535 https://doi.org/10.1007/s10535-007-0069-x text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0069-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chung H. Institute of Plant and Microbial Biology, Academia Sinica, 115, Taipei, Taiwan Republic of China aut NATIONALLICENCE 700 1- Chen J. Institute of Biotechnology, National University of Kaohsiung, 811, Kaohsiung, Taiwan Republic of China aut NATIONALLICENCE 700 1- Chang W. Institute of Plant and Microbial Biology, Academia Sinica, 115, Taipei, Taiwan Republic of China aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/2(2007-06-01), 346-350 0006-3134 51:2<346 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer