caa a22 4500 463171683 CHVBK 20180406164815.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s00344-005-0147-2 doi (NATIONALLICENCE)springer-10.1007/s00344-005-0147-2 The Effect of Plant Growth Regulators and Sucrose on the Micropropagation and Microtuberization of Dioscorea nipponica Makino [Elektronische Daten] [Feng-Qing Chen, Yang Fu, De-Li Wang, Xiang Gao, Li Wang] The effects of sucrose, plant growth regulators, MS (Murashige and Skoog), and ½MS salt media formulations were investigated for the development of shoot cultures, microtuber induction, and plantlet regeneration in Dioscorea nipponica. The cytokinin N-benzyladenine (BA) in the range of 0.5-2.0 mg/l showed strong enhancing effects on microtuber induction only when used in conjunction with the auxin alpha-naphthalene acetic acid (NAA), with the effect that NAA increased from 0.5 to 2.0 mg/l. Murashige and Skoog salt media supplemented with sucrose at 3% (w/v) gave the highest frequencies of shoot induction (86%) when BA was present at 2.0 mg/l and NAA at 1.0 mg/l. Sucrose at 7% (w/v) was the single most significant medium constituent for microtuber growth. The heaviest microtubers were formed on media containing 1.0 mg/l BA and 2.0 mg/l (0.073 g), especially with 7% sucrose (3.46 g). With media containing ½MS, 2% sucrose, and 0.1% (w/v) activated charcoal, the percentage of rooting was maximal when supplemented with 1.0 mg/l BA and 0.5 mg/l NAA for the in vitro produced shoots (95%) and BA and NAA both at 0.5 mg/l for the microtubers (100%). When removed from culture flasks and transferred into sterilized soil in a greenhouse, most of the hardened plantlets survived (over 91% after 1 week), and they were suitable for field planting after 1 month. Springer Science+Business Media, Inc., 2007 Dioscorea nipponica Makino nationallicence Microtuber induction nationallicence Regeneration nationallicence Hormone nationallicence Sucrose nationallicence Chen Feng-Qing Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, People's Republic of China aut Fu Yang Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, People's Republic of China aut Wang De-Li Key Laboratory of Vegetation Ecology, Ministry of Education, 130024, Changchun, People's Republic of China aut Gao Xiang Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, People's Republic of China aut Wang Li Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, People's Republic of China aut Journal of Plant Growth Regulation Springer-Verlag; www.springer-ny.com 26/1(2007-03-01), 38-45 0721-7595 26:1<38 2007 26 344 https://doi.org/10.1007/s00344-005-0147-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00344-005-0147-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Feng-Qing Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Fu Yang Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Wang De-Li Key Laboratory of Vegetation Ecology, Ministry of Education, 130024, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Gao Xiang Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Wang Li Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Plant Growth Regulation Springer-Verlag; www.springer-ny.com 26/1(2007-03-01), 38-45 0721-7595 26:1<38 2007 26 344 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer