caa a22 4500 463171691 CHVBK 20180406164815.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s00344-006-0029-2 doi (NATIONALLICENCE)springer-10.1007/s00344-006-0029-2 Responses of Chrysanthemum Cells to Mechanical Stimulation Require Intact Microtubules and Plasma Membrane-Cell Wall Adhesion [Elektronische Daten] [Jing Zhou, Bochu Wang, Yi Li, Yichuan Wang, Liqing Zhu] Plant cells are highly susceptible and receptive to physical factors, both in nature and under experimental conditions. Exposure to mechanical forces dramatically results in morphological and microstructural alterations in their growth. In the present study, cells from chrysanthemum (Dendranthema morifolium) were subjected to constant pressure from an agarose matrix, which surrounded and immobilized the cells to form a cell-gel block. Cells in the mechanically loaded blocks elongated and divided, with an axis preferentially perpendicular to the direction of principal stress vectors. After a sucrose-induced plasmolysis, application of peptides containing an RGD motif, which interferes with plasma membrane-cell wall adhesion, reduced the oriented growth under stress conditions. Moreover, colchicines, but not cytochalasin B, abolished the effects of mechanical stress on cell morphology. Cellulose staining revealed that mechanical force reinforces the architecture of cell walls and application of mechanical force, and RGD peptides caused aggregative staining on the surface of plasmolyzed protoplasts. These results provide evidence that the oriented cell growth in response to compressive stress requires the maintenance of plasmalemma-cell wall adhesion and intact microtubules. Stress-triggered wall development in individual plant cells was also demonstrated. Springer Science+Business Media, Inc., 2007 Mechanical force nationallicence Dendranthema morifolium nationallicence Plasma membrane-cell wall adhesion nationallicence RGD peptides nationallicence Microtubules nationallicence Zhou Jing Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, 400044, Chongqing, People's Republic of China aut Wang Bochu Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, 400044, Chongqing, People's Republic of China aut Li Yi Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, 400044, Chongqing, People's Republic of China aut Wang Yichuan Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, 400044, Chongqing, People's Republic of China aut Zhu Liqing Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, 400044, Chongqing, People's Republic of China aut Journal of Plant Growth Regulation Springer-Verlag; www.springer-ny.com 26/1(2007-03-01), 55-68 0721-7595 26:1<55 2007 26 344 https://doi.org/10.1007/s00344-006-0029-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00344-006-0029-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Jing Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, 400044, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Bochu Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, 400044, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Li Yi Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, 400044, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yichuan Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, 400044, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Liqing Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Bioengineering College, Chongqing University, 400044, Chongqing, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Plant Growth Regulation Springer-Verlag; www.springer-ny.com 26/1(2007-03-01), 55-68 0721-7595 26:1<55 2007 26 344 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer