caa a22 4500 463171756 CHVBK 20180406164815.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s00344-007-9015-6 doi (NATIONALLICENCE)springer-10.1007/s00344-007-9015-6 A Bacterial Indole-3-acetyl-L-aspartic Acid Hydrolase Inhibits Mung Bean ( Vigna radiata L.) Seed Germination Through Arginine-rich Intracellular Delivery [Elektronische Daten] [Kevin Liu, Han-Jung Lee, Sio Leong, Chen-Lun Liu, Jyh-Ching Chou] Indole-3-acetyl-L-aspartic acid (IAA-Asp) is a natural product in many plant species and plays many important roles in auxin metabolism and plant physiology. IAA-Asp hydrolysis activity is, therefore, believed to affect plant physiology through changes in IAA metabolism in plants. We applied a newly discovered technique, arginine-rich intracellular delivery (AID), to deliver a bacterial IAA-Asp hydrolase into cells of mung bean (Vigna radiata) seeds and measured its effects on mung bean seed germination. IAA-Asp hydrolase inhibited seed germination about 12 h after the enzyme was delivered into cells of mung bean seeds both covalently and noncovalently. Mung bean seed germination was delayed by 36 h when the enzyme protein was noncovalently attached to the AID peptide and longer than 60 h when the enzyme protein was covalently attached to the AID peptide. Root elongation of mung bean plants was inhibited as much as 90% or 80%, respectively, when the IAA-Asp hydrolase was delivered with the AID peptide by covalent or noncovalent association. Further thin-layer chromatography analysis of plant extracts indicated that the levels of IAA increased about 12 h after treatment and reached their peak at 24 h. This result suggests that IAA-Asp hydrolase may increase IAA levels and inhibit seed germination of mung bean plants and that the AID peptide is a new, rapid, and efficient experimental tool to study the in vivo activity of enzymes of interest in plant cells. Springer Science+Business Media, LLC, 2007 Hydrolase nationallicence IAA nationallicence IAA conjugate nationallicence Intracellular delivery nationallicence Mung bean nationallicence Seed germination nationallicence Vigna radiata nationallicence Liu Kevin Department of Life Science, National Dong Hwa University, 97401, Shou-feng, Hualien, Taiwan aut Lee Han-Jung Department of Life Science, National Dong Hwa University, 97401, Shou-feng, Hualien, Taiwan aut Leong Sio Department of Life Science, National Dong Hwa University, 97401, Shou-feng, Hualien, Taiwan aut Liu Chen-Lun Department of Life Science, National Dong Hwa University, 97401, Shou-feng, Hualien, Taiwan aut Chou Jyh-Ching Department of Life Science, National Dong Hwa University, 97401, Shou-feng, Hualien, Taiwan aut Journal of Plant Growth Regulation Springer-Verlag 26/3(2007-09-01), 278-284 0721-7595 26:3<278 2007 26 344 https://doi.org/10.1007/s00344-007-9015-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00344-007-9015-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Kevin Department of Life Science, National Dong Hwa University, 97401, Shou-feng, Hualien, Taiwan aut NATIONALLICENCE 700 1- Lee Han-Jung Department of Life Science, National Dong Hwa University, 97401, Shou-feng, Hualien, Taiwan aut NATIONALLICENCE 700 1- Leong Sio Department of Life Science, National Dong Hwa University, 97401, Shou-feng, Hualien, Taiwan aut NATIONALLICENCE 700 1- Liu Chen-Lun Department of Life Science, National Dong Hwa University, 97401, Shou-feng, Hualien, Taiwan aut NATIONALLICENCE 700 1- Chou Jyh-Ching Department of Life Science, National Dong Hwa University, 97401, Shou-feng, Hualien, Taiwan aut NATIONALLICENCE 773 0- Journal of Plant Growth Regulation Springer-Verlag 26/3(2007-09-01), 278-284 0721-7595 26:3<278 2007 26 344 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer