caa a22 4500 463171764 CHVBK 20180406164815.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s00344-007-9012-9 doi (NATIONALLICENCE)springer-10.1007/s00344-007-9012-9 Role of cAMP in Gibberellin Promotion of Seed Germination in Orobanche minor Smith [Elektronische Daten] [Kimio Uematsu, Masatoshi Nakajima, Isomaro Yamaguchi, Koichi Yoneyama, Yasuhisa Fukui] Adenosine 3′,5′-cyclic monophosphate (cAMP) is known as a key second messenger in many living organisms, regulating a wide range of cellular responses. In higher plants the function of cAMP is poorly understood. In this study, we examined the role of cAMP in seed germination of the root parasitic plant Orobanche minor whose seeds require preincubation in warm moist environments for several days, termed conditioning, prior to exposure to germination stimulants released from roots of host plants. Accumulation of endogenous cAMP was observed in the conditioned O. minor seeds. When the seeds were exposed to light or supraoptimal temperature during the conditioning period, cAMP did not accumulate and the seeds showed low germination rates after stimulation with strigol, a germination stimulant. Addition of membrane-permeable cAMP to the medium restored the germination rates of the seeds treated with light or supraoptimal temperature during the conditioning period, suggesting that cAMP functions during the conditioning period. The endogenous cAMP levels of the seeds conditioned in the light or at a supraoptimal temperature were elevated by treatment of the seeds with gibberellin (GA) during the conditioning period. Uniconazole, a potent inhibitor of GA biosynthesis, blocked elevation of the cAMP level. Furthermore, a correlation between the endogenous cAMP level and GA level was observed during the conditioning period. These results suggest that GAs elevate the cAMP level, which is required for the germination of O. minor seeds. Springer Science+Business Media, LLC, 2007 cAMP nationallicence Cyclic nucleotide nationallicence Seed germination nationallicence Gibberellin nationallicence Parasitic plant · Orobanche minor nationallicence Uematsu Kimio Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Tokyo, Bunkyo-ku, Japan aut Nakajima Masatoshi Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Tokyo, Bunkyo-ku, Japan aut Yamaguchi Isomaro Maebashi Institute of Technology, 371-0816, Maebashi, Gunma, Japan aut Yoneyama Koichi Weed Science Center, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut Fukui Yasuhisa Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Tokyo, Bunkyo-ku, Japan aut Journal of Plant Growth Regulation Springer-Verlag 26/3(2007-09-01), 245-254 0721-7595 26:3<245 2007 26 344 https://doi.org/10.1007/s00344-007-9012-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00344-007-9012-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Uematsu Kimio Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Tokyo, Bunkyo-ku, Japan aut NATIONALLICENCE 700 1- Nakajima Masatoshi Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Tokyo, Bunkyo-ku, Japan aut NATIONALLICENCE 700 1- Yamaguchi Isomaro Maebashi Institute of Technology, 371-0816, Maebashi, Gunma, Japan aut NATIONALLICENCE 700 1- Yoneyama Koichi Weed Science Center, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut NATIONALLICENCE 700 1- Fukui Yasuhisa Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, 113-8657, Tokyo, Bunkyo-ku, Japan aut NATIONALLICENCE 773 0- Journal of Plant Growth Regulation Springer-Verlag 26/3(2007-09-01), 245-254 0721-7595 26:3<245 2007 26 344 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer