caa a22 4500 463171837 CHVBK 20180406164815.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s00344-007-9007-6 doi (NATIONALLICENCE)springer-10.1007/s00344-007-9007-6 Factors Affecting Somatic Embryogenesis Induction and Conversion in "Paradise Tree” ( Melia azedarach L.) [Elektronische Daten] [Silvia Vila, Hebe Rey, Luis Mroginski] Factors affecting somatic embryogenesis induction and conversion in paradise tree (Melia azedarach) were evaluated. Somatic embryogenesis was influenced by plant growth regulators, explant stage, carbohydrate source and concentration, gelling agents, light, and induction times. MS medium with 4.54 μM thidiazuron (TDZ) was optimal for the induction of embryogenic tissue. Zygotic embryos that were 1-1.5 mm long (torpedo and early cotyledonal stage) had a greater embryogenic response than smaller or larger embryos and better conversion of somatic embryos into plants. In general, embryos that formed in medium containing 1% or 5% carbohydrate were hyperhydrics or fused, respectively, whereas those that formed in medium with a carbohydrate concentration of 3% had better morphology. Raffinose at 3% yielded satisfactory somatic embryo induction with good morphology and the best values of conversion into plants. Induction and conversion of somatic embryos were superior on medium solidified with agar A-1296. The explants maintained under 160μmol m−2 s−1 or 1 week in darkness and later 160μmol m−2 s−1 produced a significantly higher embryogenic index. Only 4 days of treatment on induction medium, with either raffinose or sucrose at 3% as a carbohydrate source, were required to induce somatic embryogenesis, but longer exposure, until 18 days, increased the yield and improved the morphology of somatic embryos. Springer Science+Business Media, LLC, 2007 Paradise tree nationallicence Somatic embryogenesis nationallicence Explants stage nationallicence Carbohydrate source nationallicence Vila Silvia Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE), Casilla de Correos 209, 3400, Corrientes, Argentina aut Rey Hebe Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE), Casilla de Correos 209, 3400, Corrientes, Argentina aut Mroginski Luis Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE), Casilla de Correos 209, 3400, Corrientes, Argentina aut Journal of Plant Growth Regulation Springer-Verlag 26/3(2007-09-01), 268-277 0721-7595 26:3<268 2007 26 344 https://doi.org/10.1007/s00344-007-9007-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00344-007-9007-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vila Silvia Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE), Casilla de Correos 209, 3400, Corrientes, Argentina aut NATIONALLICENCE 700 1- Rey Hebe Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE), Casilla de Correos 209, 3400, Corrientes, Argentina aut NATIONALLICENCE 700 1- Mroginski Luis Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE), Casilla de Correos 209, 3400, Corrientes, Argentina aut NATIONALLICENCE 773 0- Journal of Plant Growth Regulation Springer-Verlag 26/3(2007-09-01), 268-277 0721-7595 26:3<268 2007 26 344 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer