caa a22 4500 463171861 CHVBK 20180406164815.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s00344-007-9023-6 doi (NATIONALLICENCE)springer-10.1007/s00344-007-9023-6 Hormone Profiling by LC-QToF-MS/MS in Dormant Macadamia integrifolia [Elektronische Daten] Correlations with Abnormal Vertical Growth [Andrew Fletcher, Johanna Mader] A method for analyzing multiple plant hormone groups in small samples with a complex matrix was developed to initiate a study of the physiology of abnormal vertical growth (AVG) in Macadamia integrifolia (cv. HAES344). Cytokinins (CKs), gibberellins (GAs), abscisic acid (ABA), and auxins were detected in xylem sap and apical and lateral buds using high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (LC-QToF-MS/MS). The extraction method separated compounds with high sensitivity in positive (CKs) and negative (ABA, auxins, GAs) modes of QToF-MS/MS. CK profiles differed in xylem sap and apical and lateral buds irrespective of AVG symptoms. Trans-zeatin riboside (t-ZR) was dominant in sap of normal and AVG trees (∼4 and 6 pmol g−1 FW, respectively). In apical buds isopentenyl adenine (iP) (∼30 pmol g−1 FW) was the most abundant CK, and in lateral buds trans-zeatin (t-Z) (22-24 pmol g−1 FW) and iP (24-30 pmol g−1 FW) were the most abundant. t-Z levels of AVG trees were higher in apical buds (13.88 vs. 6.6 pmol g−1 FW, p<0.05) and lower in sap (0.16 vs. 0.51 pmol ml−1, p<0.005) compared to normal trees. ABA in lateral buds was 1.9 times higher (p<0.001) in AVG. IAA was below quantification, whereas indole-3-butyric acid (IBA) was consistently present. GA7 was the dominant GA in apical and lateral buds of all trees (100-150 pmol g−1 FW). GA3, 4, & 9 were consistently present at low concentrations (<12 pmol g−1 FW) in buds. GAs1, 3, & 9 were detected in xylem sap at low concentrations (<0.5 pmol g−1 FW). Differences in sap amino acids (AA) were also assessed. In sap from AVG trees, asparagine and glutamine increased significantly (p<0.05) in their contribution to total AA. Potential AVG hormone correlations are discussed. Springer Science+Business Media, LLC, 2007 Cytokinins nationallicence Auxins nationallicence Gibberellins nationallicence ABA nationallicence Amino acids nationallicence Apical nationallicence Lateral nationallicence Bud nationallicence Xylem sap nationallicence Fletcher Andrew School of Integrative Biology, University of Queensland, John Hines Building, Mansfield Place, St. Lucia, 4072, Brisbane, Queensland, Australia aut Mader Johanna School of Integrative Biology, University of Queensland, John Hines Building, Mansfield Place, St. Lucia, 4072, Brisbane, Queensland, Australia aut Journal of Plant Growth Regulation Springer-Verlag 26/4(2007-12-01), 351-361 0721-7595 26:4<351 2007 26 344 https://doi.org/10.1007/s00344-007-9023-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00344-007-9023-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fletcher Andrew School of Integrative Biology, University of Queensland, John Hines Building, Mansfield Place, St. Lucia, 4072, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Mader Johanna School of Integrative Biology, University of Queensland, John Hines Building, Mansfield Place, St. Lucia, 4072, Brisbane, Queensland, Australia aut NATIONALLICENCE 773 0- Journal of Plant Growth Regulation Springer-Verlag 26/4(2007-12-01), 351-361 0721-7595 26:4<351 2007 26 344 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer