caa a22 4500 463175042 CHVBK 20180406164823.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s10336-006-0094-0 doi (NATIONALLICENCE)springer-10.1007/s10336-006-0094-0 Haemosporidian infection in passerine birds from Lower Saxony [Elektronische Daten] [S. Wiersch, T. Lubjuhn, W. Maier, H. Kampen] Blood samples from 94 coal tits (Parus ater), 56 great tits (Parus major) and 219 pied flycatchers (Ficedula hypoleuca), caught between 1993 and 2002 at two localities in Lower Saxony, Germany, were examined for haemosporidian infection by parasite-specific polymerase chain reaction (PCR). A simple PCR targeting the 18 SSU rRNA gene of the parasites was used for rapid screening of the samples and generated a total infection prevalence of 20.6% (76/369): 6.8% (n=15) of the pied flycatchers, 19.1% (n=18) of the coal tits and 76.8% (n=43) of the great tits were infected. The positive specimens were re-examined by a cytochrome b gene-directed nested PCR producing significantly longer DNA fragments (approx. 520bp) that were sequenced and analysed against GenBank-deposited nucleotide sequences. In various numbers (once to 30 times), a total of 13 parasitic DNA sequences differing from 2.9 to 8.5% (13-45 nucleotides) were demonstrated in the three bird species. Due to similarities of 98-100% with GenBank entries, 11 sequences could be assigned to Plasmodium sp. and two to the genus Haemoproteus. In summary, 57 birds were infected with Plasmodium and 19 with Haemoproteus, corresponding to 15.4 and 5.1% of all birds examined, and to 75 and 25% of all birds tested positive. As the only defined species, Haemoproteus majoris was identified in 17 great tits. Dt. Ornithologen-Gesellschaft e.V., 2006 Avian malaria nationallicence Cytochrome b gene sequences nationallicence Infection prevalence nationallicence Lower Saxony nationallicence Passeriformes nationallicence Wiersch S. Institute for Medical Microbiology, Immunology and Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut Lubjuhn T. Institute for Evolutionary Biology and Ecology, University of Bonn, An der Immenburg 1, 53121, Bonn, Germany aut Maier W. Institute for Medical Microbiology, Immunology and Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut Kampen H. Institute for Medical Microbiology, Immunology and Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut Journal of Ornithology Springer-Verlag 148/1(2007-01-01), 17-24 0021-8375 148:1<17 2007 148 10336 https://doi.org/10.1007/s10336-006-0094-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10336-006-0094-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wiersch S. Institute for Medical Microbiology, Immunology and Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut NATIONALLICENCE 700 1- Lubjuhn T. Institute for Evolutionary Biology and Ecology, University of Bonn, An der Immenburg 1, 53121, Bonn, Germany aut NATIONALLICENCE 700 1- Maier W. Institute for Medical Microbiology, Immunology and Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut NATIONALLICENCE 700 1- Kampen H. Institute for Medical Microbiology, Immunology and Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany aut NATIONALLICENCE 773 0- Journal of Ornithology Springer-Verlag 148/1(2007-01-01), 17-24 0021-8375 148:1<17 2007 148 10336 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer