caa a22 4500 463175794 CHVBK 20180406164825.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10336-007-0217-2 doi (NATIONALLICENCE)springer-10.1007/s10336-007-0217-2 Gonadotropin-inhibitory hormone in seasonally-breeding songbirds: neuroanatomy and functional biology [Elektronische Daten] [George Bentley, Nicole Perfito, Takayoshi Ubuka, Kazuyoshi Ukena, Tomohiro Osugi, Sara O'Brien, Kazuyoshi Tsutsui, John Wingfield] The discovery of gonadotropin-inhibitory hormone (GnIH) in Japanese Quail Coturnix japonica has given us a new perspective on the neuroendocrine control of reproductive physiology and behavior. Using highly photoperiodic passerine songbirds as our model system, we have been able to investigate GnIH neuroanatomy and functional biology. The distribution of GnIH peptide is similar among the galliform and passerine species studied so far. We have identified GnIH cDNA and localized its transcript in Gambel's White-crowned Sparrow Zonotrichia leucophrys gambelii. There is a high degree of homology between the galliform and passerine GnIH cDNA sequences and peptides. Based on transcript localization, GnIH precursor polypeptide is only produced in the paraventricular nucleus, yet GnIH-immunoreactive fibers project to multiple brain areas, suggesting multiple physiological and behavioral functions. GnIH immunoreactive neurons appear to contact gonadotropin-releasing hormone (GnRH)-I and -II neurons, raising the possibility of direct regulation of GnRH by GnIH. In a photoperiod experiment, GnIH neurons increased in area during the termination of reproduction in Song Sparrows, Melospiza melodia, implying temporal photoperiodic regulation of this neuropeptide. Using peripheral administration of GnIH in vivo, we demonstrated rapid inhibition of LH release in laboratory and field experiments. Furthermore, we sought to determine the effects of central infusions of GnIH. Centrally-administered GnIH rapidly reduced circulating LH and significantly reduced the number of copulation solicitations performed in response to song playback, with no reduction of locomotor activity. Central infusion of rhodaminated GnIH elucidated putative GnIH binding sites in the median eminence close to GnRH-I fiber terminals, and in the midbrain on or close to GnRH-II neurons. These data demonstrate direct effects of GnIH upon reproductive physiology and behavior, possibly at multiple physiological levels and over different time-frames. Thus, the discovery of GnIH has unearthed a complex and interesting system for the regulation of avian reproductive biology. Dt. Ornithologen-Gesellschaft e.V., 2007 Gonadotropins nationallicence Hypothalamus nationallicence Luteinizing hormone nationallicence Pituitary nationallicence Reproduction nationallicence Bentley George Department of Integrative Biology and The Helen Wills Neuroscience Institute, University of California, #3140, 3060 Valley Life Sciences Building, 94720-3140, Berkeley, CA, USA aut Perfito Nicole Department of Integrative Biology and The Helen Wills Neuroscience Institute, University of California, #3140, 3060 Valley Life Sciences Building, 94720-3140, Berkeley, CA, USA aut Ubuka Takayoshi Department of Integrative Biology and The Helen Wills Neuroscience Institute, University of California, #3140, 3060 Valley Life Sciences Building, 94720-3140, Berkeley, CA, USA aut Ukena Kazuyoshi Laboratory of Brain Science, Faculty of Integrated Arts and Sciences, Hiroshima University, 739-8521, Higashi-Hiroshima, Japan aut Osugi Tomohiro Laboratory of Integrative Brain Sciences, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1-6-1 Nishi-Waseda, Shinjuku-ku, 169-8050, Tokyo, Japan aut O'Brien Sara Department of Biology, University of Washington, 98195, Seattle, WA, USA aut Tsutsui Kazuyoshi Laboratory of Integrative Brain Sciences, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1-6-1 Nishi-Waseda, Shinjuku-ku, 169-8050, Tokyo, Japan aut Wingfield John Department of Biology, University of Washington, 98195, Seattle, WA, USA aut Journal of Ornithology Springer-Verlag 148(2007-12-01), 521-526 0021-8375 148<521 2007 148 10336 https://doi.org/10.1007/s10336-007-0217-2 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10336-007-0217-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bentley George Department of Integrative Biology and The Helen Wills Neuroscience Institute, University of California, #3140, 3060 Valley Life Sciences Building, 94720-3140, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Perfito Nicole Department of Integrative Biology and The Helen Wills Neuroscience Institute, University of California, #3140, 3060 Valley Life Sciences Building, 94720-3140, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Ubuka Takayoshi Department of Integrative Biology and The Helen Wills Neuroscience Institute, University of California, #3140, 3060 Valley Life Sciences Building, 94720-3140, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Ukena Kazuyoshi Laboratory of Brain Science, Faculty of Integrated Arts and Sciences, Hiroshima University, 739-8521, Higashi-Hiroshima, Japan aut NATIONALLICENCE 700 1- Osugi Tomohiro Laboratory of Integrative Brain Sciences, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1-6-1 Nishi-Waseda, Shinjuku-ku, 169-8050, Tokyo, Japan aut NATIONALLICENCE 700 1- O'Brien Sara Department of Biology, University of Washington, 98195, Seattle, WA, USA aut NATIONALLICENCE 700 1- Tsutsui Kazuyoshi Laboratory of Integrative Brain Sciences, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1-6-1 Nishi-Waseda, Shinjuku-ku, 169-8050, Tokyo, Japan aut NATIONALLICENCE 700 1- Wingfield John Department of Biology, University of Washington, 98195, Seattle, WA, USA aut NATIONALLICENCE 773 0- Journal of Ornithology Springer-Verlag 148(2007-12-01), 521-526 0021-8375 148<521 2007 148 10336 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer