caa a22 4500 463175875 CHVBK 20180406164825.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10336-007-0225-2 doi (NATIONALLICENCE)springer-10.1007/s10336-007-0225-2 Discovery of gonadotropin-inhibitory hormone in a domesticated bird, its mode of action and functional significance [Elektronische Daten] [Kazuyoshi Tsutsui, Takayoshi Ubuka, Hong Yin, Tomohiro Osugi, Kazuyoshi Ukena, George Bentley, Peter Sharp, John Wingfield] Neuropeptide control of gonadotropin secretion at the level of the anterior pituitary gland is primarily through the stimulatory action of the hypothalamic decapeptide gonadotropin-releasing hormone (GnRH) in vertebrates. Until recently, no neuropeptide that directly inhibits gonadotropin secretion had been identified. In 2000, we discovered a novel hypothalamic dodecapeptide that directly inhibits gonadotropin release in quail and termed it gonadotropin-inhibitory hormone (GnIH). A gonadotropin inhibitory system is an intriguing concept and provides us with an unprecedented opportunity to study the regulation of avian reproduction from an entirely novel standpoint. To elucidate the mode of action of GnIH, we then identified a novel G protein-coupled receptor for GnIH in quail. The receptor possessed seven transmembrane domains and specifically bound to GnIH in a concentration-dependent manner. The GnIH receptor was found to be expressed in the pituitary and several brain regions including the hypothalamus. These results indicate that GnIH acts directly on the pituitary via GnIH receptor to inhibit gonadotropin release. GnIH may also act on the hypothalamus to inhibit GnRH release, because GnIH fibers were observed to contact GnRH cell bodies. To demonstrate the functional significance of GnIH and its potential role as a key neuropeptide involved in avian reproduction, we further investigated the action of GnIH on gonadal development and maintenance in quail. Chronic treatment with GnIH inhibited photoinduced testicular development and maintenance by decreasing gonadotropin synthesis and release. Melatonin is a key factor involved in GnIH neural function. Quail GnIH neurons were found to contain melatonin receptor and melatonin stimulated expression of GnIH mRNA and mature GnIH peptide in a dose-dependent manner. It is concluded that GnIH is capable of transducing photoperiodic information via changes in the melatonin signal and of influencing the reproductive axes of birds. Dt. Ornithologen-Gesellschaft e.V., 2007 Gonadotropins nationallicence Gonadotropin-inhibitory hormone (GnIH) nationallicence Gonadotropin-releasing hormone (GnRH) nationallicence Melatonin nationallicence Hypothalamus nationallicence Pituitary nationallicence Reproduction nationallicence Tsutsui Kazuyoshi Laboratory of Integrative Brain Sciences, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1-6-1 Nishi-Waseda, 169-8050, Tokyo, Shinjuku-ku, Japan aut Ubuka Takayoshi Department of Integrative Biology, University of California, 94720, Berkeley, CA, USA aut Yin Hong Laboratory of Integrative Brain Sciences, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1-6-1 Nishi-Waseda, 169-8050, Tokyo, Shinjuku-ku, Japan aut Osugi Tomohiro Laboratory of Integrative Brain Sciences, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1-6-1 Nishi-Waseda, 169-8050, Tokyo, Shinjuku-ku, Japan aut Ukena Kazuyoshi Laboratory of Brain Science, Faculty of Integrated Arts and Sciences, Hiroshima University, 739-8521, Higashi-Hiroshima, Japan aut Bentley George Department of Integrative Biology, University of California, 94720, Berkeley, CA, USA aut Sharp Peter Division of Genetics and Genomics, Roslin Institute, EH25 9PS, Midlothian, UK aut Wingfield John Department of Biology, University of Washington, 98195, Seattle, WA, USA aut Journal of Ornithology Springer-Verlag 148(2007-12-01), 515-520 0021-8375 148<515 2007 148 10336 https://doi.org/10.1007/s10336-007-0225-2 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10336-007-0225-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsutsui Kazuyoshi Laboratory of Integrative Brain Sciences, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1-6-1 Nishi-Waseda, 169-8050, Tokyo, Shinjuku-ku, Japan aut NATIONALLICENCE 700 1- Ubuka Takayoshi Department of Integrative Biology, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Yin Hong Laboratory of Integrative Brain Sciences, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1-6-1 Nishi-Waseda, 169-8050, Tokyo, Shinjuku-ku, Japan aut NATIONALLICENCE 700 1- Osugi Tomohiro Laboratory of Integrative Brain Sciences, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1-6-1 Nishi-Waseda, 169-8050, Tokyo, Shinjuku-ku, Japan aut NATIONALLICENCE 700 1- Ukena Kazuyoshi Laboratory of Brain Science, Faculty of Integrated Arts and Sciences, Hiroshima University, 739-8521, Higashi-Hiroshima, Japan aut NATIONALLICENCE 700 1- Bentley George Department of Integrative Biology, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Sharp Peter Division of Genetics and Genomics, Roslin Institute, EH25 9PS, Midlothian, UK aut NATIONALLICENCE 700 1- Wingfield John Department of Biology, University of Washington, 98195, Seattle, WA, USA aut NATIONALLICENCE 773 0- Journal of Ornithology Springer-Verlag 148(2007-12-01), 515-520 0021-8375 148<515 2007 148 10336 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer