caa a22 4500 463180178 CHVBK 20180406164836.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s00381-007-0345-2 doi (NATIONALLICENCE)springer-10.1007/s00381-007-0345-2 Diffuse alterations in synaptic protein expression following focal traumatic brain injury in the immature rat [Elektronische Daten] [G. Gobbel, C. Bonfield, E. Carson-Walter, P. Adelson] Introduction: The mechanisms responsible for cognitive decline after traumatic brain injury (TBI) in pediatric patients are poorly understood. The present study examined the potential role of synaptic alterations in this process by using an animal model of immature head injury to define the impact of TBI on expression of the synaptic protein, synaptophysin. Materials and methods: After craniotomy, TBI was induced in postnatal day17 (PND17) rats using controlled cortical impact delivered to the left hemisphere. NeuN, a neuronal marker, and synaptophysin expression were examined 1day, 1week, and 1month after injury by immunohistochemistry and immunoblotting. Results: There were significant decreases in both NeuN and synaptophysin after 1day and 1week but not 1month after injury within the hippocampus and neocortex adjacent to the impact site compared to sham-injured controls. The decrease in synaptophysin and NeuN was also noted in the contralateral hippocampus by 1day after injury and in the contralateral neocortex by 1week, indicating that changes in protein expression were not solely localized to the injury site but occurred in more distant regions as well. Discussion: In conclusion, the decrease and recovery in synaptophysin parallel the cognitive changes that occur after experimental TBI in the PND17 rat, which suggests that changes in this protein may contribute to cognitive declines after injury. The results also suggest that, in spite of the focal nature of the impact, diffuse alterations in protein expression can occur after immature TBI and may contribute to the subsequent cognitive dysfunction. Springer-Verlag, 2007 Pediatric nationallicence Rat nationallicence NeuN nationallicence Synaptophysin nationallicence Traumatic brain injury nationallicence Gobbel G. Department of Neurological Surgery, University of Pittsburgh, Suite B-400, UPMC Presbyterian, 200 Lothrop Street, 15213, Pittsburgh, PA, USA aut Bonfield C. Department of Neurological Surgery, University of Pittsburgh, Suite B-400, UPMC Presbyterian, 200 Lothrop Street, 15213, Pittsburgh, PA, USA aut Carson-Walter E. Department of Neurological Surgery, University of Pittsburgh, Suite B-400, UPMC Presbyterian, 200 Lothrop Street, 15213, Pittsburgh, PA, USA aut Adelson P. Department of Neurological Surgery, University of Pittsburgh, Suite B-400, UPMC Presbyterian, 200 Lothrop Street, 15213, Pittsburgh, PA, USA aut Child's Nervous System Springer-Verlag 23/10(2007-10-01), 1171-1179 0256-7040 23:10<1171 2007 23 381 https://doi.org/10.1007/s00381-007-0345-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00381-007-0345-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gobbel G. Department of Neurological Surgery, University of Pittsburgh, Suite B-400, UPMC Presbyterian, 200 Lothrop Street, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Bonfield C. Department of Neurological Surgery, University of Pittsburgh, Suite B-400, UPMC Presbyterian, 200 Lothrop Street, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Carson-Walter E. Department of Neurological Surgery, University of Pittsburgh, Suite B-400, UPMC Presbyterian, 200 Lothrop Street, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Adelson P. Department of Neurological Surgery, University of Pittsburgh, Suite B-400, UPMC Presbyterian, 200 Lothrop Street, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 773 0- Child's Nervous System Springer-Verlag 23/10(2007-10-01), 1171-1179 0256-7040 23:10<1171 2007 23 381 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer