caa a22 4500 463185412 CHVBK 20180406164851.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s00726-006-0349-3 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0349-3 Modulation of an AMPA-like glutamate receptor (SqGluR) gating by L- and D-aspartic acids [Elektronische Daten] [E. R. Brown, S. Piscopo, J.-T. Chun, M. Francone, I. Mirabile, A. D'Aniello] Summary.: L- and D-aspartic acids (L-Asp and D-Asp) are present in the majority of nervous systems. In phylogeny, significant levels have been reported in mollusc brains, particularly cephalopods. To examine the role of L- and D-Asp on a cephalopod receptor, we studied ligand gating of a squid glutamate receptor (SqGluR) expressed in HEK 239 (human embryonic kidney) cells. Under voltage clamp, application of L-glutamate (L-Glu; 1-30 mM), but not D-glutamate (D-Glu), or L- or D-Asp, evoked an inward current of 0.1 nA. L- or D-Asp (200 µM) applied with 20 mM L-Glu, slowed the time course of activation and inactivation of the L-Glu gated current (time constant increased from 1 s (L-Glu alone) to 3 s (D-Asp and L-Glu) and to 19 s (L-Asp and L-Glu)). Our results suggest that in molluscan systems, aspartic acid could act as a neuromodulator during glutamatergic transmission and could significantly alter synaptic integration by slowing glutamate receptor gating. Springer-Verlag, 2006 Keywords: Glutamate receptor - SqGluR - Heterologous expression - L- and D-aspartic acid - Neuromodulation nationallicence Brown E. R. Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut Piscopo S. Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut Chun J.-T Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut Francone M. Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut Mirabile I. Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut D'Aniello A. Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut Amino Acids Springer-Verlag 32/1(2007-01-01), 53-57 0939-4451 32:1<53 2007 32 726 https://doi.org/10.1007/s00726-006-0349-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0349-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brown E. R. Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut NATIONALLICENCE 700 1- Piscopo S. Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut NATIONALLICENCE 700 1- Chun J.-T Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut NATIONALLICENCE 700 1- Francone M. Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut NATIONALLICENCE 700 1- Mirabile I. Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut NATIONALLICENCE 700 1- D'Aniello A. Neurobiology Laboratory, Stazione Zoologica ‘Anton Dohrn', Naples, Italy aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 32/1(2007-01-01), 53-57 0939-4451 32:1<53 2007 32 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer