caa a22 4500 46318551X CHVBK 20180406164851.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s00726-006-0381-3 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0381-3 Protective effect of gamma-aminobutyric acid (GABA) against cytotoxicity of ethanol in isolated rat hepatocytes involves modulations in cellular polyamine levels [Elektronische Daten] [T. Norikura, A. Kojima-Yuasa, D. Opare Kennedy, I. Matsui-Yuasa] Summary.: Gamma-aminobutyric acid (GABA) is considered to be a multifunctional molecule with various physiological effects throughout the body. It is also evident that the liver contains GABA and its transporter. However, the functions of GABA in liver have not been well documented. In this study, the cytoprotective effect of GABA against ethanol-induced hepatotoxicity was evaluated in primary cultured rat hepatocytes. Addition of ethanol induced decrease of cell viability in a dose-dependent manner. However, treatment with GABA resulted in a dose-dependent recovery from ethanol (150 mM)-induced cytotoxicity. GABA reversed the ethanol-induced decrease in intracellular polyamine levels. Furthermore, the addition of polyamines also reversed the ethanol-induced decrease of cell viability. These results suggest that GABA is protective against the cytotoxicity of ethanol in isolated rat hepatocytes and this effect may be modulated by the maintenance of intracellular polyamine levels. Springer-Verlag, 2006 Keywords: GABA - Ethanol - Hepatocytes - Polyamines nationallicence Norikura T. Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan aut Kojima-Yuasa A. Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan aut Opare Kennedy D. Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan aut Matsui-Yuasa I. Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan aut Amino Acids Springer-Verlag 32/3(2007-04-01), 419-423 0939-4451 32:3<419 2007 32 726 https://doi.org/10.1007/s00726-006-0381-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0381-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Norikura T. Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan aut NATIONALLICENCE 700 1- Kojima-Yuasa A. Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan aut NATIONALLICENCE 700 1- Opare Kennedy D. Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan aut NATIONALLICENCE 700 1- Matsui-Yuasa I. Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 32/3(2007-04-01), 419-423 0939-4451 32:3<419 2007 32 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer