caa a22 4500 463185528 CHVBK 20180406164851.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s00726-006-0382-2 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0382-2 Protective effect of taurine on respiratory burst activity of polymorphonuclear leukocytes in endotoxemia [Elektronische Daten] [M. Ekremoğlu, N. Türközkan, H. Erdamar, Y. Kurt, H. Yaman] Summary.: The aim of this study was to evaluate the effect of endotoxin on PMN leukocyte respiratory burst activity by measuring G6PD, NADPH oxidase and XO activities in guinea pig. In addition, the possible protective role of taurine against endotoxin-mediated PMN leukocyte function was examined. All experiments were performed with four groups (control, taurine, endotoxemia, taurine plus endotoxin) of ten guinea pigs. After the endotoxin was administrated (4 mg/kg) both G6PD and NADPH oxidase activities were significantly reduced compared with the control group. NADPH oxidase activity returned to the control value and G6PD activity also increased but it did not reach the control value. However when taurine was administrated (300 mg/kg) the activity of NADPH oxidase reached the control value; furthermore, G6PD activity also increased but it could not reach to the control value. When taurine was administrated alone, no effect on these enzymes was observed. Following the endotoxin administration, the activity of XO considerably increased. When taurine was administrated together with endotoxine and alone, this activity decreased compared to control value in both conditions. These results indicate that the O2 •− formation in PMN leukocytes after the endotoxin administration is ensured by the catalysis of XO due to the inhibited NADPH oxidase activity. It was observed that taurine has considerable anti-inflammatory and antioxidant effects. However, conflicting results were obtained when taurine was administrated alone or together with an oxidant agent. Springer-Verlag, 2006 Keywords: Respiratory burst - Polymorphonuclear leukocyte - Taurine - Endotoxemia nationallicence ROS : reactive oxygen species nationallicence O2 •− : superoxide nationallicence G6PD : glucose-6-phosphate dehydrogenase nationallicence NADPH : nicotinamide adenine dinucleotide phosphate nationallicence NO : nitric oxide nationallicence XO : xanthine oxidase nationallicence PMN : polymorphonuclear nationallicence LPS : lipopolysaccharide nationallicence ip : intraperitoneally nationallicence iNOS : inducible nitric oxide synthase nationallicence ONOO− : peroxynitrite nationallicence Ekremoğlu M. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut Türközkan N. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut Erdamar H. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut Kurt Y. Department of Biochemistry, Gulhane School of Medicine, Ankara, Turkey aut Yaman H. Department of Biochemistry, Gulhane School of Medicine, Ankara, Turkey aut Amino Acids Springer-Verlag 32/3(2007-04-01), 413-417 0939-4451 32:3<413 2007 32 726 https://doi.org/10.1007/s00726-006-0382-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0382-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ekremoğlu M. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut NATIONALLICENCE 700 1- Türközkan N. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut NATIONALLICENCE 700 1- Erdamar H. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut NATIONALLICENCE 700 1- Kurt Y. Department of Biochemistry, Gulhane School of Medicine, Ankara, Turkey aut NATIONALLICENCE 700 1- Yaman H. Department of Biochemistry, Gulhane School of Medicine, Ankara, Turkey aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 32/3(2007-04-01), 413-417 0939-4451 32:3<413 2007 32 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer