caa a22 4500 463185692 CHVBK 20180406164852.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s00726-006-0430-y doi (NATIONALLICENCE)springer-10.1007/s00726-006-0430-y Sulfenic acid in human serum albumin [Elektronische Daten] [S. Carballal, B. Alvarez, L. Turell, H. Botti, B. A. Freeman, R. Radi] Summary.: Sulfenic acid (RSOH) is a central intermediate in both the reversible and irreversible redox modulation by reactive species of an increasing number of proteins involved in signal transduction and enzymatic pathways. In this paper we focus on human serum albumin (HSA), the most abundant plasma protein, proposed to serve antioxidant functions in the vascular compartment. Sulfenic acid in HSA has been previously detected using different methods after oxidation of its single free thiol Cys34 through one- or two-electron mechanisms. Since recent evidence suggests that sulfenic acid in HSA is stabilized within the protein environment, this derivative represents an appropriate model to examine protein sulfenic acid biochemistry, structure and reactivity. Sulfenic acid in HSA could be involved in mixed disufide formation, supporting a role of HSA-Cys34 as an important redox regulator in extracellular compartments. Springer-Verlag, 2006 Keywords: Thiol - Human serum albumin - Sulfenic acid - Peroxynitrite - Hydrogen peroxide - Free radicals nationallicence HSA : human serum albumin nationallicence HSA-SH : the thiol of HSA nationallicence BSA : bovine serum albumin nationallicence SH/HSA : amount of thiol per albumin molecule nationallicence RSOH : sulfenic acid nationallicence NBD-Cl : 7-chloro-4-nitrobenzo-2-oxa-1,3-diazol nationallicence DTNB : 5,5′-dithio-bis(2-nitrobenzoic acid) nationallicence dimedone : 5,5-dimethyl-1,3-cyclohexanedione nationallicence GSH : glutathione nationallicence GSSG : glutathione disulfide nationallicence Carballal S. Laboratorio de Enzimología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay aut Alvarez B. Laboratorio de Enzimología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay aut Turell L. Laboratorio de Enzimología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay aut Botti H. Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay aut Freeman B. A. Department of Pharmacology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA aut Radi R. Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay aut Amino Acids Springer-Verlag 32/4(2007-05-01), 543-551 0939-4451 32:4<543 2007 32 726 https://doi.org/10.1007/s00726-006-0430-y text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0430-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carballal S. Laboratorio de Enzimología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay aut NATIONALLICENCE 700 1- Alvarez B. Laboratorio de Enzimología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay aut NATIONALLICENCE 700 1- Turell L. Laboratorio de Enzimología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay aut NATIONALLICENCE 700 1- Botti H. Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay aut NATIONALLICENCE 700 1- Freeman B. A. Department of Pharmacology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA aut NATIONALLICENCE 700 1- Radi R. Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 32/4(2007-05-01), 543-551 0939-4451 32:4<543 2007 32 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer