caa a22 4500 463185838 CHVBK 20180406164852.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s00726-006-0429-4 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0429-4 EPR spin-trapping of protein radicals to investigate biological oxidative mechanisms [Elektronische Daten] [O. Augusto, S. Muntz Vaz] Summary.: Presently, free radicals and oxidants are considered to mediate from signaling circuits involved in physiology and pathology to cell and tissue injury. The elucidation of these many inter-related processes requires a better understanding of cellular oxidative mechanisms many of which are mediated by protein radicals. Here, we will discuss the potentialities of EPR spin-trapping of protein radicals to unravel oxidative mechanisms. An overview of the methodology and its application to identify protein residues that are the target of specific oxidants, characterize emerging oxidants, and discriminate radical from non radical mechanisms will be presented. The examples are based on work developed in our laboratories but will be discussed in a broad scenario to emphasize that simple experiments can provide relevant insights into the biological reactivity of known and emerging biological oxidants and into signaling mechanisms. Springer-Verlag, 2006 Keywords: Oxidative mechanisms - Protein radicals - EPR - EPR spin-trapping nationallicence BSA : bovine serum albumin nationallicence Cu,Zn-SOD : superoxide dismutase nationallicence DBNBS : 3,5-dibromo-4-nitrosobenzenesulfonic acid nationallicence DMPO : 5,5-dimethylpyrroline N-oxide nationallicence EPR : electron paramagnetic resonance nationallicence ESI : electrospray ionization nationallicence GSH : glutathione nationallicence Hb : hemoglobin nationallicence HCO4 − : peroxycarbonate or peroxymonocarbonate nationallicence HSA : human serum albumin nationallicence LC : liquid chromatography nationallicence MALDI-TOF : matrix-assisted laser desorption/ionization time of flight nationallicence MNP : 2-methyl-2-nitrosopropane nationallicence MS : mass spectrometry nationallicence PBN : phenyl-N-t-butylnitrone nationallicence Peroxynitrite : sum of peroxynitrite anion (ONOO−, oxoperoxonitrate (−1)) and peroxynitrous acid (ONOOH, hydrogen oxoperoxonitrate) unless specified nationallicence RNS : reactive nitrogen species nationallicence ROS : reactive oxygen species nationallicence Augusto O. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil aut Muntz Vaz S. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil aut Amino Acids Springer-Verlag 32/4(2007-05-01), 535-542 0939-4451 32:4<535 2007 32 726 https://doi.org/10.1007/s00726-006-0429-4 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0429-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Augusto O. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Muntz Vaz S. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 32/4(2007-05-01), 535-542 0939-4451 32:4<535 2007 32 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer