caa a22 4500 463185951 CHVBK 20180406164853.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s00726-006-0409-8 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0409-8 Free amino acid and dipeptide changes in the body fluids from Alzheimer's disease subjects [Elektronische Daten] [A. N. Fonteh, R. J. Harrington, A. Tsai, P. Liao, M. G. Harrington] Summary.: Our aim was to determine changes in free amino acid (FAA) and dipeptide (DP) concentrations in probable Alzheimer's disease (pAD) subjects compared with control (CT) subjects using liquid chromatography and electrospray ionization tandem mass spectrometry (LCMS2). We recruited gender- and age-matched study participants based on neurological and neuropsychological assessments. We measured FAAs and DPs in cerebrospinal fluid (CSF), plasma and urine using LCMS2 with selected reaction monitoring (SRM). Imidazole-containing FAAs (histidine, methyl-histidine), catecholamines (L-DOPA and dopamine), citrulline, ornithine, glycine and antioxidant DPs (carnosine and anserine) accounted for the major changes between CT and pAD. Carnosine levels were significantly lower in pAD (328.4 ± 91.31 nmol/dl) than in CT plasma (654.23 ± 100.61 nmol/dl). In contrast, L-DOPA levels were higher in pAD (1400.84 ± 253.68) than CT (513.10 ± 121.61 nmol/dl) plasma. These data underscore the importance of FAA and DP metabolism in the pathogenesis of AD. Since our data show changes in antioxidants, neurotransmitters and their precursors, or FAA associated with urea metabolism in pAD compared with CT, we propose that manipulation of these metabolic pathways may be important in preventing AD progression. Springer-Verlag, 2006 Keywords: Amino acids - Dipeptides - Tandem mass spectrometry - Cerebrospinal fluid - Plasma - Alzheimer's disease nationallicence CT : control study participants nationallicence pAD : probable Alzheimer's disease study participants nationallicence CT_M : control male nationallicence CT_F : control female nationallicence pAD_M : pAD male nationallicence pAD_F : pAD female nationallicence MMSE : mini-mental state examination nationallicence ADAS-cog : Alzheimer's Disease Assessment Scale- cognitive subscale nationallicence LCMS2 : liquid chromatography electrospray ionization tandem mass spectrometry nationallicence SRM : selected reaction monitoring nationallicence FAA : free amino acid nationallicence DP : dipeptide nationallicence GABA : γ-aminobutyric acid nationallicence βABA : β-aminobutyric acid nationallicence ABA : α-aminobutyric acid nationallicence DABA : 2,4-diaminobutyric acid nationallicence DOPA : 3,4-dihydroxyphenyl-L-alanine nationallicence Fonteh A. N. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, U.S.A. aut Harrington R. J. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, U.S.A. aut Tsai A. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, U.S.A. aut Liao P. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, U.S.A. aut Harrington M. G. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, U.S.A. aut Amino Acids Springer-Verlag 32/2(2007-02-01), 213-224 0939-4451 32:2<213 2007 32 726 https://doi.org/10.1007/s00726-006-0409-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0409-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fonteh A. N. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, U.S.A aut NATIONALLICENCE 700 1- Harrington R. J. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, U.S.A aut NATIONALLICENCE 700 1- Tsai A. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, U.S.A aut NATIONALLICENCE 700 1- Liao P. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, U.S.A aut NATIONALLICENCE 700 1- Harrington M. G. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, CA, U.S.A aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 32/2(2007-02-01), 213-224 0939-4451 32:2<213 2007 32 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer