caa a22 4500 463186036 CHVBK 20180406164853.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s00726-006-0358-2 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0358-2 Dietary γ-aminobutyric acid affects the brain protein synthesis rate in young rats [Elektronische Daten] [K. Tujioka, S. Okuyama, H. Yokogoshi, Y. Fukaya, K. Hayase, K. Horie, M. Kim] Summary.: The purpose of this study was to determine whether the γ-aminobutyric acid (GABA) affects the rate of brain protein synthesis in male rats. Two experiments were done on five or three groups of young rats (5 wk) given the diets containing 20% casein administrated 0 mg, 25 mg, 50 mg, 100 mg or 200 mg/100 g body weight GABA dissolved in saline by oral gavage for 1 day (d) (Experiment 1), and given the diets contained 0%, 0.25% or 0.5% GABA added to the 20% casein diet (Experiment 2) for 10 d. The plasma concentration of growth hormone (GH) was the highest in rats administrated 50 mg and 100 mg/100 g body weight GABA. The concentration of serum GABA increased significantly with the supplementation groups. The fractional (Ks) rates of protein synthesis in brain regions, liver and gastrocnemius muscle increased significantly with the 20% casein + 0.25% GABA diet and still more 20% casein + 0.5% GABA compared with the 20% casein diet. In brain regions, liver and gastrocnemius muscle, the RNA activity [g protein synthesized/(g RNA·d)] significantly correlated with the fractional rate of protein synthesis. The RNA concentration (mg RNA/g protein) was not related to the fractional rate of protein synthesis in any organ. Our results suggest that the treatment of GABA to young male rats are likely to increase the concentrations of plasma GH and the rate of protein synthesis in the brain, and that RNA activity is at least partly related to the fractional rate of brain protein synthesis. Springer-Verlag, 2006 Keywords: γ-Aminobutyric acid - Growth hormone - Protein synthesis - Brain - Rats nationallicence GABA : γ-aminobutyric acid nationallicence GH : growth hormone nationallicence d : days nationallicence Tujioka K. Laboratory of Nutritional Biochemistry, School of Food and Nutritional Sciences, COE Program in the 21st Century, The University of Shizuoka, Yada, Shizuoka, Japan aut Okuyama S. Laboratory of Nutritional Biochemistry, School of Food and Nutritional Sciences, COE Program in the 21st Century, The University of Shizuoka, Yada, Shizuoka, Japan aut Yokogoshi H. Laboratory of Nutritional Biochemistry, School of Food and Nutritional Sciences, COE Program in the 21st Century, The University of Shizuoka, Yada, Shizuoka, Japan aut Fukaya Y. Department of Home Economics, Aichi University of Education, Kariya, Aichi, Japan aut Hayase K. Department of Home Economics, Aichi University of Education, Kariya, Aichi, Japan aut Horie K. Research Department, Pharma Foods International Company Ltd., Kyoto, Japan aut Kim M. Research Department, Pharma Foods International Company Ltd., Kyoto, Japan aut Amino Acids Springer-Verlag 32/2(2007-02-01), 255-260 0939-4451 32:2<255 2007 32 726 https://doi.org/10.1007/s00726-006-0358-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0358-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tujioka K. Laboratory of Nutritional Biochemistry, School of Food and Nutritional Sciences, COE Program in the 21st Century, The University of Shizuoka, Yada, Shizuoka, Japan aut NATIONALLICENCE 700 1- Okuyama S. Laboratory of Nutritional Biochemistry, School of Food and Nutritional Sciences, COE Program in the 21st Century, The University of Shizuoka, Yada, Shizuoka, Japan aut NATIONALLICENCE 700 1- Yokogoshi H. Laboratory of Nutritional Biochemistry, School of Food and Nutritional Sciences, COE Program in the 21st Century, The University of Shizuoka, Yada, Shizuoka, Japan aut NATIONALLICENCE 700 1- Fukaya Y. Department of Home Economics, Aichi University of Education, Kariya, Aichi, Japan aut NATIONALLICENCE 700 1- Hayase K. Department of Home Economics, Aichi University of Education, Kariya, Aichi, Japan aut NATIONALLICENCE 700 1- Horie K. Research Department, Pharma Foods International Company Ltd., Kyoto, Japan aut NATIONALLICENCE 700 1- Kim M. Research Department, Pharma Foods International Company Ltd., Kyoto, Japan aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 32/2(2007-02-01), 255-260 0939-4451 32:2<255 2007 32 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer