caa a22 4500 463186117 CHVBK 20180406164853.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s00726-006-0408-9 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0408-9 A facile HPLC method for optical purity and quantitative measurements of phenylalanine from the hydrolyzed aspartame under different pH and temperature after its derivatization with a fluorescent reagent [Elektronische Daten] [T.-J. Hsien, S. Chen] Summary.: In this paper, the artificial sweetener aspartame is deliberately hydrolyzed under different pH and temperature in the matrix, and time period for the hydrolysis. The HPLC analysis is then performed to quantitatively measure the amount and the optical purity of phenylalanine produced as a result of hydrolysis in the matrix after its functionalization with a fluorescent reagent. The results show that the amount of phenylalanine in the matrix is affected by the pH variation during the hydrolysis and found increased in low pH conditions. High temperature or long time periods for the decomposition also increases the amount, which indicates that beverages and foods containing aspartame as a sweetener may not be safe for phenylketonuria patients to consume if they are stored under these conditions. Conversely, the optical purity of phenylalanine, expressed as the percentage of d-enantiomer, is not affected by pH variations. However, it decreases as the length of time elapsed is increased or surrounding temperature is elevated during the decomposition. Springer-Verlag, 2006 Keywords: Aspartame - Optical purity - Phenylalanine - Teicoplanin - Fluorescent reagent nationallicence Hsien T.-J Department of Applied Chemistry, National Chiayi University, Chiayi, Taiwan, China aut Chen S. Department of Applied Chemistry, National Chiayi University, Chiayi, Taiwan, China aut Amino Acids Springer-Verlag 33/1(2007-07-01), 123-128 0939-4451 33:1<123 2007 33 726 https://doi.org/10.1007/s00726-006-0408-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0408-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hsien T.-J Department of Applied Chemistry, National Chiayi University, Chiayi, Taiwan, China aut NATIONALLICENCE 700 1- Chen S. Department of Applied Chemistry, National Chiayi University, Chiayi, Taiwan, China aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/1(2007-07-01), 123-128 0939-4451 33:1<123 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer