caa a22 4500 463186125 CHVBK 20180406164853.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s00726-006-0417-8 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0417-8 The apoptotic effect of intercalating agents on HPV-negative cervical cancer C-33A cells [Elektronische Daten] [Yun Hee Kang, Kyung-Ac Lee, Young Yang, Soo-Hyun Kim, Joo Hwan Kim, Sue Nie Park, Sang-Gi Paik, Do-Young Yoon] Summary.: Cervical cancer is one of the leading causes of female cancer death worldwide with about 500,000 deaths per year. Both mitomycin C and cisplatin are alkylating agents, which bind and intercalate DNA, and thus used as anti-cancer drugs. In these studies, we focused on investigating the apoptotic effects of intercalating agents on HPV-negative cervical cancer C-33A cells. Accordingly, C-33A cells were treated with carboplatin, mitomycin C or cisplatin. Cell cycle analysis revealed that treatment with mitomycin C and cisplatin but not with carboplatin resulted in apoptosis. Both mitomycin C and cisplatin induced apoptosis in C-33A cells via caspase-8 and -3 processing in a Fas/FasL-dependent manner and also suppressed IL-18 expression, while they down-regulated IκB expression and up-regulated p65 expression. These results suggest that both mitomycin C and cisplatin induce apoptosis, not only via the caspase-8 and -3 dependent Fas/FasL pathway, but also via the regulation of NF-κB activity and IL-18 expression in HPV-negative cervical cancer C-33A cells. Springer-Verlag, 2006 Keywords: IL-18 - FasL - Mitomycin C - Cisplatin - Apoptosis - Cervical cancer nationallicence IFN-γ : interferon-γ nationallicence FasL : Fas ligand nationallicence NF-κB : nuclear facor-κB nationallicence HPV : human papillomavirus nationallicence MMC : mitomycin C nationallicence CIS : cisplatin nationallicence CA : carboplatin nationallicence Kang Yun Hee Department of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea aut Lee Kyung-Ac Department of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea aut Yang Young Department of Biological Sciences, Sookmyung Women's University, Seoul, South Korea aut Kim Soo-Hyun Institute of Biomedical Science and Technology, Konkuk University, Seoul, South Korea aut Kim Joo Hwan Division of Genetic Toxicology, National Institute of Toxicological Research, Seoul, South Korea aut Park Sue Nie Division of Genetic Toxicology, National Institute of Toxicological Research, Seoul, South Korea aut Paik Sang-Gi Department of Biology, Chungnam National University, Daejeon, South Korea aut Yoon Do-Young Department of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea aut Amino Acids Springer-Verlag 33/1(2007-07-01), 105-112 0939-4451 33:1<105 2007 33 726 https://doi.org/10.1007/s00726-006-0417-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0417-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kang Yun Hee Department of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea aut NATIONALLICENCE 700 1- Lee Kyung-Ac Department of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea aut NATIONALLICENCE 700 1- Yang Young Department of Biological Sciences, Sookmyung Women's University, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Soo-Hyun Institute of Biomedical Science and Technology, Konkuk University, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Joo Hwan Division of Genetic Toxicology, National Institute of Toxicological Research, Seoul, South Korea aut NATIONALLICENCE 700 1- Park Sue Nie Division of Genetic Toxicology, National Institute of Toxicological Research, Seoul, South Korea aut NATIONALLICENCE 700 1- Paik Sang-Gi Department of Biology, Chungnam National University, Daejeon, South Korea aut NATIONALLICENCE 700 1- Yoon Do-Young Department of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/1(2007-07-01), 105-112 0939-4451 33:1<105 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer