caa a22 4500 463186168 CHVBK 20180406164853.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s00726-006-0423-x doi (NATIONALLICENCE)springer-10.1007/s00726-006-0423-x A fluorescent electrophilic reagent, 9-fluorenone-4-carbonyl chloride (FCC), for the enantioresolution of amino acids on a teicoplanin phase under the elution of the methanol-based solvent mixture [Elektronische Daten] [T.-J. Hsien, S. Chen] Summary.: A fluorescent electrophilic reagent, 9-fluorenone-4-carbonyl chloride (FCC), is chosen to functionalize amino acids in alkaline medium before their HPLC resolution. FCC reacts with both primary and secondary amino acids to produce stable and highly fluorescent derivatives suitable for sensitive and efficient chromatographic determination and resolution on a teicoplanin chiral stationary phase (CSP) using the methanol-based solvent mixture as the mobile phase. The detection limit is in the picomole range and approximately 0.01% of the d-enantiomer in an excess of the l-enantiomer is detectable. However, the resolution is not reproducible under the elution of either the water- or the acetonitrile-based mobile phase. The increase in solubility of analyte in the mobile phase seems to be responsible. Upon comparison under the optimal chromatographic conditions, the resolution is better than that for the 9-fluorenylmethyl chloroformate (FMOC) or 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatives reported previously. Springer-Verlag, 2006 Keywords: Fluorescent tagging reagent - Column liquid chromatography - Enantioresolution - Amino acids - Teicoplanin - Trace enantiomeric impurities - Enantiomeric ratio nationallicence Hsien T.-J Department of Applied Chemistry, National Chiayi University, Chiayi, Taiwan, China aut Chen S. Department of Applied Chemistry, National Chiayi University, Chiayi, Taiwan, China aut Amino Acids Springer-Verlag 33/1(2007-07-01), 97-104 0939-4451 33:1<97 2007 33 726 https://doi.org/10.1007/s00726-006-0423-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0423-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hsien T.-J Department of Applied Chemistry, National Chiayi University, Chiayi, Taiwan, China aut NATIONALLICENCE 700 1- Chen S. Department of Applied Chemistry, National Chiayi University, Chiayi, Taiwan, China aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/1(2007-07-01), 97-104 0939-4451 33:1<97 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer