caa a22 4500 463186230 CHVBK 20180406164853.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s00726-006-0400-4 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0400-4 The time-profile of the PBMC HSP70 response to in vitro heat shock appears temperature-dependent [Elektronische Daten] [R. Lovell, L. Madden, S. Carroll, L. McNaughton] Summary.: Heat shock proteins (HSPs) are synthesised by cells subsequent to a stress exposure and are known to confer protection to the cell in response to a second challenge. HSP induction and decay are correlated to thermotolerance and may therefore be used as a biomarker of thermal history. The current study tested the temperature-dependent nature of the heat shock response and characterised its time profile of induction. Whole blood from 6 healthy males (Age: 26 ± (SD) 2 yrs; Body mass 74.2 ± 3.8 kgs; VO2max: 49.1 ± 4.0 ml·kg−1·min−1) were isolated and exposed to in vitro heat shock (HS) at 37, 38, 39, 40, and 41 °C for a period of 90 min. After HS the temperature was returned to 37 °C and intracellular HSP70 was quantified from the leukocytes at 0, 2, 4, and 6 h after heat treatment. The concentration of HSP70 was not different between temperatures (P > 0.05), but the time-profile of HSP70 synthesis appeared temperature-dependent. At control (37 °C) and lower temperatures (38-39 °C) the mean HSP70 concentration increased up to 4 h post HS (P < 0.05) and then returned towards baseline values by 6 h post HS. With in vitro hyperthermic conditions (40-41 °C), the time-profile was characterised by a sharp rise in HSP70 levels immediately after treatment (P < 0.05 for 40 °C at 0 h), followed by a progressive decline over time. The results suggest a temperature-dependent time-profile of HSP70 synthesis. In addition, the temperature at which HSP70 is inducted might be lower than 37 °C. Springer-Verlag, 2006 Keywords: Heat shock protein 70 - Temperature - In vitro, PBMCs nationallicence Lovell R. Department of Sport, Health and Exercise Science, University of Hull, Hull, U.K. aut Madden L. Post-Graduate Medical Institute, University of Hull, Hull, U.K. aut Carroll S. Department of Sport, Health and Exercise Science, University of Hull, Hull, U.K. aut McNaughton L. Department of Sport, Health and Exercise Science, University of Hull, Hull, U.K. aut Amino Acids Springer-Verlag 33/1(2007-07-01), 137-144 0939-4451 33:1<137 2007 33 726 https://doi.org/10.1007/s00726-006-0400-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0400-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lovell R. Department of Sport, Health and Exercise Science, University of Hull, Hull, U.K aut NATIONALLICENCE 700 1- Madden L. Post-Graduate Medical Institute, University of Hull, Hull, U.K aut NATIONALLICENCE 700 1- Carroll S. Department of Sport, Health and Exercise Science, University of Hull, Hull, U.K aut NATIONALLICENCE 700 1- McNaughton L. Department of Sport, Health and Exercise Science, University of Hull, Hull, U.K aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/1(2007-07-01), 137-144 0939-4451 33:1<137 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer