caa a22 4500 463186249 CHVBK 20180406164853.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s00726-006-0455-2 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0455-2 Effect of the L- or D-aspartate on ecto-5′nucleotidase activity and on cellular viability in cultured neurons: participation of the adenosine A2A receptors [Elektronische Daten] [C. R. Boeck, E. H. Kroth, M. J. Bronzatto, D. Vendite] Summary.: Glutamate increases the extracellular adenosine levels, an important endogenous neuromodulator. The neurotoxicity induced by glutamate increases the ecto-5′-nucleotidase activity in neurons, which produces adenosine from AMP. L- and D-aspartate (Asp) mimic most of the actions of glutamate in the N-methyl-D-aspartate (NMDA) receptors. In the present study, both amino acids stimulated the ecto-5′-nucleotidase activity in cerebellar granule cells. MK-801 and AP-5 prevented the L- and D-Asp-evoked activation of ecto-5′-nucleotidase. Both NMDA receptor antagonists prevented completely the damage induced by L-Asp, but partially the D-Asp-induced damage. The antagonist of adenosine A2A receptors (ZM 241385) prevented totally the L- Asp-induced cellular death, but partially the neurotoxicity induced by D-Asp and the antagonist of adenosine A1 receptors (CPT) had no effect. The results indicated a different involvement of NMDA receptors on the L- or D-Asp-evoked activation of ecto-5′-nucleotidase and on cellular damage. The adenosine formed from ecto-5′-nucleotidase stimulation preferentially acted on adenosine A2A receptor which is probably co-operating with the neurotoxicity induced by amino acids. Springer-Verlag, 2007 Keywords: L- and D-aspartate - Neurotoxicity - Ecto-5′-nucleotidase - Adenosine receptors nationallicence Boeck C. R. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, Brazil aut Kroth E. H. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Bronzatto M. J. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Vendite D. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Amino Acids Springer-Verlag 33/3(2007-09-01), 439-444 0939-4451 33:3<439 2007 33 726 https://doi.org/10.1007/s00726-006-0455-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0455-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boeck C. R. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, Brazil aut NATIONALLICENCE 700 1- Kroth E. H. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Bronzatto M. J. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Vendite D. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/3(2007-09-01), 439-444 0939-4451 33:3<439 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer