caa a22 4500 463186311 CHVBK 20180406164854.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s00726-006-0452-5 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0452-5 Purification of branched-chain amino acid aminotransferase from Helicobacter pylori NCTC 11637 [Elektronische Daten] [M. Saito, K. Nishimura, S. Wakabayashi, T. Kurihara, Y. Nagata] Summary.: Branched-chain amino acid aminotransferase was purified by several column chromatographies from Helicobacter pylori NCTC 11637, and the N-terminal amino acid sequence was analyzed. The enzyme gene was sequenced based on a putative branched-chain amino acid aminotransferase gene, ilvE of H. pylori 26695, and the whole amino acid sequence was deduced from the nucleotide sequence. The enzyme existed in a homodimer with a calculated subunit molecular weight (MW) of 37,539 and an isoelectric point (pI) of 6.47. The enzyme showed high affinity to 2-oxoglutarate (K m = 0.085 mM) and L-isoleucine (K m = 0.34 mM), and V max was 27.3 µmol/min/mg. The best substrate was found to be L-isoleucine followed by L-leucine and L-valine. No activity was shown toward the D-enantiomers of these amino acids. The optimal pH and temperature were pH 8.0 and 37 °C, respectively. Springer-Verlag, 2006 Keywords: Branched-chain amino acid aminotransferase - Purification - Helicobacter pylori - ilvE gene nationallicence Saito M. Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Tokyo, Japan aut Nishimura K. Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Tokyo, Japan aut Wakabayashi S. Department of Life Sciences, Graduate School of Life Science, University of Hyogo, Kamigohri, Hyogo, Japan aut Kurihara T. Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan aut Nagata Y. Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Tokyo, Japan aut Amino Acids Springer-Verlag 33/3(2007-09-01), 445-449 0939-4451 33:3<445 2007 33 726 https://doi.org/10.1007/s00726-006-0452-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0452-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saito M. Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Tokyo, Japan aut NATIONALLICENCE 700 1- Nishimura K. Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Tokyo, Japan aut NATIONALLICENCE 700 1- Wakabayashi S. Department of Life Sciences, Graduate School of Life Science, University of Hyogo, Kamigohri, Hyogo, Japan aut NATIONALLICENCE 700 1- Kurihara T. Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan aut NATIONALLICENCE 700 1- Nagata Y. Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Tokyo, Japan aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/3(2007-09-01), 445-449 0939-4451 33:3<445 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer