caa a22 4500 463186400 CHVBK 20180406164854.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s00726-006-0460-5 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0460-5 Applications and current challenges of proteomic approaches, focusing on two-dimensional electrophoresis [Elektronische Daten] [F. G. G. Vercauteren, L. Arckens, R. Quirion] Summary.: Since the formulation of the concept of "proteomics” in 1995, a plethora of proteomic technologies have been developed in order to study proteomes of tissues, cells and organelles. The powerful new technologies enabled by proteomic approaches have lead to the application of these methods to an exponentially increasing variety of biological questions for highly complex protein mixtures. Continuous technical optimization allows for an ever-increasing sensitivity of proteomic techniques. In this review, a brief overview of currently available proteomic techniques and their applications is given, followed by a more detailed description of advantages and technical challenges of two-dimensional electrophoresis (2-DE). Some solutions to circumvent currently encountered technical difficulties for 2-DE analyses are proposed. Springer-Verlag, 2006 Keywords: Proteomics - Two-dimensional electrophoresis - Mass spectrometry - Applications - Shortcomings nationallicence ASB-14 : Amidosulfobetaine-14 nationallicence CHAPS : 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate nationallicence 2-DE : two-dimensional electrophoresis nationallicence DIGE : difference gel electrophoresis nationallicence HUPO : human proteome organization nationallicence IEF : isoelectric focusing nationallicence SDS-PAGE : sodium dodecyl sulfate polyacrylamide gel electrophoresis nationallicence Vercauteren F. G. G. Douglas Hospital Research Center, Department of Psychiatry, McGill University, Montréal, Québec, Canada aut Arckens L. Laboratory of Neuroplasticity and Neuroproteomics, Katholieke Universiteit Leuven, Leuven, Belgium aut Quirion R. Douglas Hospital Research Center, Department of Psychiatry, McGill University, Montréal, Québec, Canada aut Amino Acids Springer-Verlag 33/3(2007-09-01), 405-414 0939-4451 33:3<405 2007 33 726 https://doi.org/10.1007/s00726-006-0460-5 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0460-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vercauteren F. G. G. Douglas Hospital Research Center, Department of Psychiatry, McGill University, Montréal, Québec, Canada aut NATIONALLICENCE 700 1- Arckens L. Laboratory of Neuroplasticity and Neuroproteomics, Katholieke Universiteit Leuven, Leuven, Belgium aut NATIONALLICENCE 700 1- Quirion R. Douglas Hospital Research Center, Department of Psychiatry, McGill University, Montréal, Québec, Canada aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/3(2007-09-01), 405-414 0939-4451 33:3<405 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer