caa a22 4500 463186451 CHVBK 20180406164854.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00726-007-0543-y doi (NATIONALLICENCE)springer-10.1007/s00726-007-0543-y The effect of taurine on polymorphonuclear leukocyte functions in endotoxemia [Elektronische Daten] [H. Erdamar, N. Türközkan, M. Ekremoğlu, Y. Kurt, H. Yaman] Summary.: The aim of the present study was to measure MPO activity in PMN leukocytes after endotoxin administration, and to compare the levels of NO2 − competing with taurine for reaction with HOCl. Furthermore we aimed to determine TauCl levels, a product of MPO-H2O2-Halide system, and to evaluate anti-inflammatory properties of PMN in endotoxemia. In addition, our second objective was to investigate the effect of taurine, an antioxidant amino acid, on anti-bactericidal and anti-inflammatory functions of PMN after administration of endotoxin together with taurine. All experiments were performed with four groups (control, taurine, endotoxemia, and taurine plus endotoxin) of ten guinea pigs. After endotoxin administration (4 mg/kg), MPO activities increased and taurine levels decreased. Therefore levels of TauCl, NO2 •− increased. We observed the effects of taurine as conflicting. When taurine was administrated alone (300 mg/kg), all of these parameters decreased. Consequently, we suggested that taurine is influential in infected subjects but not on healthy ones as an antioxidative amino acid. In addition, we believe that in vivo effects of taurine may differ from those in vitro depending on its dosage. Springer-Verlag, 2007 Keywords: Polymorphonuclear leukocyte - Taurine - Myeloperoxidase - Nitric oxide nationallicence G6PD : glucose-6-phosphate dehydrogenase nationallicence iNOS : inducible nitric oxide synthase nationallicence ip : intraperitoneally nationallicence LPS : Lipopolysaccharide nationallicence NADPH : nicotinamide adenine dinucleotide phosphate nationallicence NO : nitric oxide nationallicence O2 •− : superoxide nationallicence ONOO− : peroxynitrite nationallicence PMN : polymorphonuclear nationallicence ROS : reactive oxygen species nationallicence XO : xanthine oxidase nationallicence Erdamar H. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut Türközkan N. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut Ekremoğlu M. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut Kurt Y. Department of Biochemistry, Gulhane School of Medicine, Ankara, Turkey aut Yaman H. Department of Biochemistry, Gulhane School of Medicine, Ankara, Turkey aut Amino Acids Springer-Verlag 33/4(2007-11-01), 581-585 0939-4451 33:4<581 2007 33 726 https://doi.org/10.1007/s00726-007-0543-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-007-0543-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Erdamar H. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut NATIONALLICENCE 700 1- Türközkan N. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut NATIONALLICENCE 700 1- Ekremoğlu M. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey aut NATIONALLICENCE 700 1- Kurt Y. Department of Biochemistry, Gulhane School of Medicine, Ankara, Turkey aut NATIONALLICENCE 700 1- Yaman H. Department of Biochemistry, Gulhane School of Medicine, Ankara, Turkey aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/4(2007-11-01), 581-585 0939-4451 33:4<581 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer