caa a22 4500 463186508 CHVBK 20180406164854.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00726-007-0503-6 doi (NATIONALLICENCE)springer-10.1007/s00726-007-0503-6 Analysis of methionine oxides and nitrogen-transporting amino acids in chilled and acclimated maize seedlings [Elektronische Daten] [A. D. Simonović, M. D. Anderson] Summary.: In maize seedlings, chilling causes a reduction of glutamine synthetase (GS) activity, while acclimation protects GS (manuscript submitted). Since ROS can oxidize both protein-bound and free Met to methionine sulfoxide (MSO) and further to methionine sulfone (MSO2, a GS inhibitor), it was hypothesized that the chilling-induced oxidative stress may cause accumulation of MSO and MSO2, thus contributing to the inactivation of GS. MSO2 preferentially inhibited the chloroplastic isoform, GS2. HPLC analysis of polar amino acids from coleoptiles + leaves, mesocotyls and roots of control, chilled, acclimated, acclimated and chilled and chilled and rewarmed plants revealed that free MSO and MSO2 do not accumulate after low temperature treatments. Nevertheless, acclimation significantly increased the expression of putative protein methionine sulfoxide reductase (PMSR), especially in mesocotyls. Different low temperature treatments caused complex changes in the profiles of N-transporting amino acids, Asp, Glu, Asn and Gln. Springer-Verlag, 2007 Keywords: Glutamine synthetase - Methionine oxides - N-transporting amino acids - Chilling - Acclimation - PMSR nationallicence C + l : coleoptiles + leaves nationallicence d : day nationallicence GS : glutamine synthetase nationallicence GS1 : cytosolic isoform of glutamine synthetase nationallicence GS2 : plastidic isoform of glutamine synthetase nationallicence MSN : L-methionine-S-sulfoximine nationallicence MSO : L-methionine sulfoxide nationallicence MSO2 : L-methionine sulfone nationallicence PMSR : protein methionine sulfoxide reductase nationallicence ROS : reactive oxygen species nationallicence Simonović A. D. Department of Biological Sciences, North Dakota State University, Fargo, ND, U.S.A. aut Anderson M. D. Department of Biological Sciences, North Dakota State University, Fargo, ND, U.S.A. aut Amino Acids Springer-Verlag 33/4(2007-11-01), 607-613 0939-4451 33:4<607 2007 33 726 https://doi.org/10.1007/s00726-007-0503-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-007-0503-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Simonović A. D. Department of Biological Sciences, North Dakota State University, Fargo, ND, U.S.A aut NATIONALLICENCE 700 1- Anderson M. D. Department of Biological Sciences, North Dakota State University, Fargo, ND, U.S.A aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/4(2007-11-01), 607-613 0939-4451 33:4<607 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer