caa a22 4500 463186540 CHVBK 20180406164855.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00726-007-0498-z doi (NATIONALLICENCE)springer-10.1007/s00726-007-0498-z DNA damage during glycation of lysine by methylglyoxal: assessment of vitamins in preventing damage [Elektronische Daten] [G. Suji, S. Sivakami] Summary.: Amino acids react with methylglyoxal to form advanced glycation end products. This reaction is known to produce free radicals. In this study, cleavage to plasmid DNA was induced by the glycation of lysine with methylglyoxal in the presence of iron(III). This system was found to produce superoxide as well as hydroxyl radicals. The abilities of various vitamins to prevent damage to plasmid DNA were evaluated. Pyridoxal-5-phosphate showed maximum protection, while pyridoxamine showed no protection. The protective abilities could be directly correlated to inhibition of production of hydroxyl and superoxide radicals. Pyridoxal-5-phosphate exhibited low radical scavenging ability as evaluated by its TEAC, but showed maximum protection probably by interfering in free radical production. Pyridoxamine did not inhibit free radical production. Thiamine and thiamine pyrophosphate, both showed protective effects albeit to different extents. Tetrahydrofolic acid showed better antioxidant activity than folic acid but was found to damage DNA by itself probably by superoxide generation. Springer-Verlag, 2007 Keywords: Advanced glycation end products - Diabetes - DNA - Free radicals - Glycation - Lysine - Methylglyoxal - Vitamins nationallicence ABTS : 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid nationallicence AGE : advanced glycation end product nationallicence MG : methylglyoxal nationallicence P : pyridoxine nationallicence PLP : pyridoxal-5-phosphate nationallicence PM : methylglyoxal, aminoguanidine, pyridoxamine nationallicence ROS : reactive oxygen species nationallicence T : thiamine nationallicence THF : tetrahydrofolic acid nationallicence TPP : thiamine pyrophosphate nationallicence Suji G. Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai, India aut Sivakami S. Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai, India aut Amino Acids Springer-Verlag 33/4(2007-11-01), 615-621 0939-4451 33:4<615 2007 33 726 https://doi.org/10.1007/s00726-007-0498-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-007-0498-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suji G. Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai, India aut NATIONALLICENCE 700 1- Sivakami S. Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai, India aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/4(2007-11-01), 615-621 0939-4451 33:4<615 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer