caa a22 4500 463186591 CHVBK 20180406164855.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00726-006-0486-8 doi (NATIONALLICENCE)springer-10.1007/s00726-006-0486-8 Taurine transporter is expressed in vascular smooth muscle cells [Elektronische Daten] [X.-B. Liao, X.-M. Zhou, J.-M. Li, Z.-P. Tan, L.-M. Liu, W. Zhang, H. Tan, Y. Lu, L.-Q. Yuan] Summary.: The regulation of vascular smooth muscle cells (VSMCs) function by taurine has been a subject of increasing interest and investigation, and taurine is taken up into cells through a specific transporter system, the taurine transporter (TAUT). In the present study, we examined the expression of TAUT in VSMCs and the kinetic parameters of the uptake process of TAUT in VSMCs. RT-PCR and western blot demonstrated that the mRNA and protein of TAUT was expressed in VSMCs in vitro. Immunohistochemistry using antibody for TAUT revealed the expression of this protein in rat thoracic aorta. The maximal [3H]taurine uptake rate in VSMCs was 37.75 ± 3.13 pmol/min per mg of protein, with a K m value of 5.42 ± 0.81 µM. Thus, VSMCs are able to express a functional taurine transporter. The regulation and detailed function of taurine and TAUT in VSMCs remain unclear, but our findings suggest a functional role for them in VSMCs metabolism. Springer-Verlag, 2007 Keywords: Taurine transporter - Vascular smooth muscle cells - Kinetic analysis nationallicence Liao X.-B Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut Zhou X.-M Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut Li J.-M Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut Tan Z.-P Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut Liu L.-M Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut Zhang W. Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut Tan H. Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut Lu Y. Institute of Metabolism and Endocrinology, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut Yuan L.-Q Institute of Metabolism and Endocrinology, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut Amino Acids Springer-Verlag 33/4(2007-11-01), 639-643 0939-4451 33:4<639 2007 33 726 https://doi.org/10.1007/s00726-006-0486-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-006-0486-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liao X.-B Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Zhou X.-M Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Li J.-M Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Tan Z.-P Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Liu L.-M Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Zhang W. Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Tan H. Department of Cardiothoracic Surgery, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Lu Y. Institute of Metabolism and Endocrinology, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Yuan L.-Q Institute of Metabolism and Endocrinology, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/4(2007-11-01), 639-643 0939-4451 33:4<639 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer