caa a22 4500 463186648 CHVBK 20180406164855.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s00726-007-0526-z doi (NATIONALLICENCE)springer-10.1007/s00726-007-0526-z Oshima T. Institute of Environmental Microbiology, Tokyo, Japan aut Unique polyamines produced by an extreme thermophile, Thermus thermophilus [Elektronische Daten] [T. Oshima] Summary.: Recent research progress on polyamines in extreme thermophiles is reviewed. Extreme thermophiles produce two types of unique polyamines; one is longer polyamines such as caldopentamine and caldohexamine, and the other is branched polyamines such as tetrakis(3-aminopropyl)ammonium. The protein synthesis catalyzed by a cell-free extract of Thermus thermophilus, an extreme thermophile, required the presence of a polyamine and the highest activity was found in the presence of tetrakis(3-aminopropyl)ammonium. In vitro experiments, longer polyamines efficiently stabilized double stranded nucleic acids and a branched polyamine, tetrakis(3-aminropyl)ammonium, stabilized stem-and-loop structures. In T. thermophilus, polyamines are synthesized from arginine by a new metabolic pathway; arginine is converted to agmatine and then agmatine is aminopropylated to N1-aminopropylagmatine which is converted to spermidine by an enzyme coded by a gene homologous to speB (a gene for agmatinase). In this new pathway spermidine is not synthesized from putrescine. Reverse genetic studies indicated that the unique polyamines are synthesized from spermidine. Springer-Verlag, 2007 Keywords: Archaeon - Caldopentamine - Cell-free protein synthesis - Hyperthermophile - Tetrakis(3-aminopropyl)ammonium nationallicence Poly(U) : polyuridilic acid nationallicence speA : gene coding for arginine decarboxylase nationallicence speB : gene coding for agmatine ureohydrolase nationallicence speD : gene coding for S-adenosylmethinine decarboxylase nationallicence speE : gene coding for spermidine synthase or spermine synthase nationallicence speC : gene coding for ornithine decarboxylase nationallicence Tm : melting temperature nationallicence Amino Acids Springer-Verlag 33/2(2007-08-01), 367-372 0939-4451 33:2<367 2007 33 726 https://doi.org/10.1007/s00726-007-0526-z text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-007-0526-z text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Oshima T. Institute of Environmental Microbiology, Tokyo, Japan aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/2(2007-08-01), 367-372 0939-4451 33:2<367 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer