caa a22 4500 463186699 CHVBK 20180406164855.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s00726-007-0522-3 doi (NATIONALLICENCE)springer-10.1007/s00726-007-0522-3 Oxidative stress and inflammation in the pathogenesis of activated polyamine catabolism-induced acute pancreatitis [Elektronische Daten] [M. Merentie, A. Uimari, M. Pietilä, R. Sinervirta, T. A. Keinänen, J. Vepsäläinen, A. Khomutov, N. Grigorenko, K.-H. Herzig, J. Jänne, L. Alhonen] Summary.: The markers of oxidative stress and inflammation were studied in acute pancreatitis in transgenic rats exhibiting activated polyamine catabolism. In addition, the effect of bismethylspermine (Me2Spm) pretreatment, preventing pancreatitis in this model, on these mediators was investigated. Lipid peroxidation was increased at 6 and 24 h after induction of pancreatitis. These changes as well as the markedly decreased superoxide dismutase activity at 24 h were abolished by Me2Spm pretreatment. Glutathione level and catalase activity changed transiently, and the effect of Me2Spm was clear at 24 h. Serum inflammatory cytokine levels increased already at 4 h whereas NF-κB was distinctly activated only at 24 h. Me2Spm prevented the increase in TNF-α and IL-6 while it had no effect on NF-κB activation. These results show that typical inflammatory and, to a lesser degree, some oxidative stress mediators are involved and beneficially affected by the disease-ameliorating polyamine analogue in our pancreatitis model. Springer-Verlag, 2007 Keywords: Spermidine/spermine N1-acetyltransferase - Inflammatory cytokines - Lipid peroxidation - Superoxide dismutase - Catalase - Glutathione nationallicence AEBSF : 4-(2-aminoethyl)benzenesulfonyl fluoride nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence EMSA : electrophoretic mobility shift assay nationallicence GSH : reduced glutathione nationallicence GSSH : oxidized glutathione nationallicence HRP : horse radish peroxidase nationallicence IκB-α : inhibitor-κBα nationallicence IL-1β : interleukin-1β nationallicence IL-6 : interleukin-6 nationallicence IL-10 : interleukin-10 nationallicence LPO : lipid hydroperoxide nationallicence NO : nitric oxide nationallicence MDL72527 : N 1 ,N 2 -bis(2,3-butanediyl)-1,4-butanediamine nationallicence Me2Spm : bismethylspermine nationallicence NF-κB : nuclear factor-κB nationallicence PAO : polyamine oxidase nationallicence PMSF : phenylmethanesulfonyl fluoride nationallicence SOD : superoxide dismutase nationallicence SSAT : spermidine/spermine-N1-acetyltransferase nationallicence TNF-α : tumor necrosis factor-α nationallicence Merentie M. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut Uimari A. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut Pietilä M. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut Sinervirta R. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut Keinänen T. A. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut Vepsäläinen J. Department of Chemistry, University of Kuopio, Kuopio, Finland aut Khomutov A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia aut Grigorenko N. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia aut Herzig K.-H Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut Jänne J. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut Alhonen L. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut Amino Acids Springer-Verlag 33/2(2007-08-01), 323-330 0939-4451 33:2<323 2007 33 726 https://doi.org/10.1007/s00726-007-0522-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-007-0522-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Merentie M. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- Uimari A. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- Pietilä M. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- Sinervirta R. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- Keinänen T. A. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- Vepsäläinen J. Department of Chemistry, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- Khomutov A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Grigorenko N. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Herzig K.-H Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- Jänne J. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- Alhonen L. Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/2(2007-08-01), 323-330 0939-4451 33:2<323 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer