caa a22 4500 463186710 CHVBK 20180406164855.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s00726-007-0523-2 doi (NATIONALLICENCE)springer-10.1007/s00726-007-0523-2 Antizyme and antizyme inhibitor activities influence cellular responses to polyamine analogs [Elektronische Daten] [J. L. A. Mitchell, T. K. Thane, J. M. Sequeira, L. J. Marton, R. Thokala] Summary.: Close structural analogs of spermidine and spermine, polyamine mimetics, are potential chemotheraputic agents as they depress cellular polyamines required for tumor growth. Specific mimetic analogs stimulate synthesis of the regulatory protein antizyme (AZ), which not only inactivates the initial enzyme in polyamine biosynthesis but also inhibits cellular uptake of polyamines. The role of AZ induction in influencing cellular uptake of representative analogs was investigated using three analogs produced by Cellgate Inc., CGC-11047, CGC-11102, and CGC-11144, which exhibit markedly distinct AZ-inducing potential. An inverse correlation was noted between the AZ-inducing activity of a compound and the steady-state levels accumulated in cells. As some tumor cells over express AZI as a means of enhancing the polyamines required for aggressive growth, analog sensitivity was examined in transgenic CHO cells expressing exogenous antizyme inhibitor protein (AZI). Although AZI over expression increased cell sensitivity to analogs, the degree of this affect varied with the analog used. Springer-Verlag, 2007 Keywords: Polyamines - Antizyme - Antizyme inhibitor - Polyamine mimetics - Polyamine analogs nationallicence AZ : antizyme nationallicence AZI : antizyme inhibitor nationallicence CHO : Chinese Hamster Ovary cells nationallicence DFMO : difluoromethylornithine nationallicence HTC : rat hepatoma cells nationallicence ODC : ornithine decarboxylase nationallicence SPD : spermidine nationallicence SPM : spermine nationallicence Mitchell J. L. A. Department of Biological Sciences, Northern Illinois University, DeKalb, IL, U.S.A. aut Thane T. K. Department of Biological Sciences, Northern Illinois University, DeKalb, IL, U.S.A. aut Sequeira J. M. Department of Biological Sciences, Northern Illinois University, DeKalb, IL, U.S.A. aut Marton L. J. Cellgate Inc., Redwood City, CA, U.S.A. aut Thokala R. Department of Biological Sciences, Northern Illinois University, DeKalb, IL, U.S.A. aut Amino Acids Springer-Verlag 33/2(2007-08-01), 291-297 0939-4451 33:2<291 2007 33 726 https://doi.org/10.1007/s00726-007-0523-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-007-0523-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mitchell J. L. A. Department of Biological Sciences, Northern Illinois University, DeKalb, IL, U.S.A aut NATIONALLICENCE 700 1- Thane T. K. Department of Biological Sciences, Northern Illinois University, DeKalb, IL, U.S.A aut NATIONALLICENCE 700 1- Sequeira J. M. Department of Biological Sciences, Northern Illinois University, DeKalb, IL, U.S.A aut NATIONALLICENCE 700 1- Marton L. J. Cellgate Inc., Redwood City, CA, U.S.A aut NATIONALLICENCE 700 1- Thokala R. Department of Biological Sciences, Northern Illinois University, DeKalb, IL, U.S.A aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/2(2007-08-01), 291-297 0939-4451 33:2<291 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer