caa a22 4500 46318677X CHVBK 20180406164856.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s00726-007-0528-x doi (NATIONALLICENCE)springer-10.1007/s00726-007-0528-x Development of an expression macroarray for amine metabolism-related genes [Elektronische Daten] [P. Chaves, F. Correa-Fiz, E. Melgarejo, J. L. Urdiales, M. A. Medina, F. Sánchez-Jiménez] Summary.: Cationic amino acids are the precursors of biogenic amines, histamine from histidine, and putrescine, spermidine and spermine from arginine/ornithine (and methionine), as well as nitric oxide. These amines play important biological roles in inter- and intracellular signaling mechanisms related to inflammation, cell proliferation and neurotransmission. Biochemical and epidemiological relationships between arginine-derived products and histamine have been reported to play important roles in physiopathological problems. In this communication, we describe the construction of an expression macroarray containing more than 30 human probes for most of the key proteins involved in biogenic amines metabolisms, as well as other inflammation- and proliferation-related probes. The array has been validated on human mast HMC-1 cells. On this model, we have got further support for an inverse correlation between polyamine and histamine synthesis previously observed on murine basophilic models. These tools should also be helpful to understand the amine roles in many other inflammatory and neoplastic pathologies. Springer-Verlag, 2007 Keywords: Expression macroarray - Polyamines - Histamine - Mast cells - Inflammation nationallicence GAPDH : glyceraldehyde-3-phosphate dehydrogenase nationallicence HDC : Histidine decarboxylase nationallicence HMT : histamine N-methyl transferase nationallicence iNOS : inducible nitric oxide synthase nationallicence SAM : S-adenosyl-methionine nationallicence SMS : spermine synthase nationallicence SRM : spermidine synthase nationallicence TPS : tryptase nationallicence Chaves P. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut Correa-Fiz F. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut Melgarejo E. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut Urdiales J. L. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut Medina M. A. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut Sánchez-Jiménez F. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut Amino Acids Springer-Verlag 33/2(2007-08-01), 315-322 0939-4451 33:2<315 2007 33 726 https://doi.org/10.1007/s00726-007-0528-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-007-0528-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaves P. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut NATIONALLICENCE 700 1- Correa-Fiz F. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut NATIONALLICENCE 700 1- Melgarejo E. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut NATIONALLICENCE 700 1- Urdiales J. L. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut NATIONALLICENCE 700 1- Medina M. A. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut NATIONALLICENCE 700 1- Sánchez-Jiménez F. ProCel Lab, Department of Molecular Biology and Biochemistry, Centre for Biomedical Research on Rare Diseases (CIBERER), Faculty of Sciences, Campus Teatinos, University of Malaga, Malaga, Spain aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/2(2007-08-01), 315-322 0939-4451 33:2<315 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer