caa a22 4500 463186796 CHVBK 20180406164856.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s00726-007-0536-x doi (NATIONALLICENCE)springer-10.1007/s00726-007-0536-x Anticancer drugs and hyperthermia enhance cytotoxicity induced by polyamine enzymatic oxidation products [Elektronische Daten] [M. Marra, E. Agostinelli, G. Tempera, A. Lombardi, G. Meo, A. Budillon, A. Abbruzzese, G. Giuberti, M. Caraglia] Summary.: A correlation between regulation of cell proliferation and polyamine metabolism is described. The latter can enter protein synthesis through the modification of eukaryotic initiation factor 5A (eIF5A) and the formation of the peculiar amino acid hypusine. Specific inhibitors of hypusine formation induce apoptosis that can be potentiated by the combination with cytokines such as interferonα (IFNα) that itself decreases hypusine synthesis. We have also demonstrated that the concomitant treatment of cancer cells with IFNα and the protein synthesis inhibitor fusion protein TGFα/Pseudomonas Aeruginosa toxin synergize in inducing cancer cell growth inhibition. Another way used by polyamines to induce apoptosis is the generation of intracellular oxidative stress through the interaction with bovine serum amine oxidase (BSAO). This enzyme used simultaneously to spermine induces apoptosis, necrosis, inhibition of cell proliferation and inhibition of DNA and protein synthesis in several cell types. The enzymatic oxidation products of polyamine, H2O2 and aldehyde(s) cause these effects. We have recently found that the cytotoxicity of anti-cancer agents, either etoposide or docetaxel, in cancer cells is potentiated in the presence of BSAO/Spermine. In conclusion, polyamine metabolites could be useful in the design of new therapeutic strategies. Springer-Verlag, 2007 Keywords: Amine oxidase - Polyamines - Multidrug resistance - Eukaryotic initiation factor 5A - Docetaxel - IFNα nationallicence AO : amine oxidase nationallicence BSA : bovine serum albumin nationallicence BSAO : bovine serum amine oxidase nationallicence CHO : chinese hamster ovary nationallicence cyclin/cdks : cyclin-dependent kinase nationallicence DFMO : 2-(difluoromethyl)ornithine nationallicence DTX : docetaxel nationallicence EGF-R : epidermal growth factor receptor nationallicence eIF5A : eukaryotic initiation factor 5A nationallicence HTC : hepatoma tissue culture nationallicence IFNα : interferon alpha nationallicence MDL 72527 : N1,N4-bis(2,3-butadienyl)-1,4-butanediamine nationallicence MDR : multidrug resistance nationallicence ODC : ornithine decarboxylase nationallicence PAO : polyamine oxidase nationallicence PEG : poly ethylene glycol nationallicence Put : putrescine nationallicence ROS : reactive oxygen species nationallicence SAMDC : S-adenosylmethionine decarboxylase nationallicence SAO : serum amine oxidase nationallicence Spd : spermidine nationallicence Spm : spermine nationallicence SSAT : spermidine/spermine N 1-acetyltransferase nationallicence VP16 : etoposide nationallicence Marra M. Department of Experimental Oncology, Experimental Pharmacology Unit, National Institute of Tumours "Fondaz. G. Pascale”, Naples, Italy aut Agostinelli E. Department of Biochemical Sciences "A. Rossi Fanelli”, Institute of Molecular Biology and Pathology, University of Rome "La Sapienza” and CNR, Rome, Italy aut Tempera G. Department of Biochemical Sciences "A. Rossi Fanelli”, Institute of Molecular Biology and Pathology, University of Rome "La Sapienza” and CNR, Rome, Italy aut Lombardi A. Department of Biochemistry and Biophysics, Second University of Naples, Naples, Italy aut Meo G. Department of Experimental Oncology, Experimental Pharmacology Unit, National Institute of Tumours "Fondaz. G. Pascale”, Naples, Italy aut Budillon A. Department of Experimental Oncology, Experimental Pharmacology Unit, National Institute of Tumours "Fondaz. G. Pascale”, Naples, Italy aut Abbruzzese A. Department of Biochemistry and Biophysics, Second University of Naples, Naples, Italy aut Giuberti G. Department of Biochemistry and Biophysics, Second University of Naples, Naples, Italy aut Caraglia M. Department of Experimental Oncology, Experimental Pharmacology Unit, National Institute of Tumours "Fondaz. G. Pascale”, Naples, Italy aut Amino Acids Springer-Verlag 33/2(2007-08-01), 273-281 0939-4451 33:2<273 2007 33 726 https://doi.org/10.1007/s00726-007-0536-x text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-007-0536-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marra M. Department of Experimental Oncology, Experimental Pharmacology Unit, National Institute of Tumours "Fondaz. G. Pascale”, Naples, Italy aut NATIONALLICENCE 700 1- Agostinelli E. Department of Biochemical Sciences "A. Rossi Fanelli”, Institute of Molecular Biology and Pathology, University of Rome "La Sapienza” and CNR, Rome, Italy aut NATIONALLICENCE 700 1- Tempera G. Department of Biochemical Sciences "A. Rossi Fanelli”, Institute of Molecular Biology and Pathology, University of Rome "La Sapienza” and CNR, Rome, Italy aut NATIONALLICENCE 700 1- Lombardi A. Department of Biochemistry and Biophysics, Second University of Naples, Naples, Italy aut NATIONALLICENCE 700 1- Meo G. Department of Experimental Oncology, Experimental Pharmacology Unit, National Institute of Tumours "Fondaz. G. Pascale”, Naples, Italy aut NATIONALLICENCE 700 1- Budillon A. Department of Experimental Oncology, Experimental Pharmacology Unit, National Institute of Tumours "Fondaz. G. Pascale”, Naples, Italy aut NATIONALLICENCE 700 1- Abbruzzese A. Department of Biochemistry and Biophysics, Second University of Naples, Naples, Italy aut NATIONALLICENCE 700 1- Giuberti G. Department of Biochemistry and Biophysics, Second University of Naples, Naples, Italy aut NATIONALLICENCE 700 1- Caraglia M. Department of Experimental Oncology, Experimental Pharmacology Unit, National Institute of Tumours "Fondaz. G. Pascale”, Naples, Italy aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/2(2007-08-01), 273-281 0939-4451 33:2<273 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer